Chitosan oligosaccharide (COS) inhibits LPS-induced inflammatory effects in RAW 264.7 macrophage cells
| Autor: | Myoung E. Moon, Haeng Soon Park, Hyun Joong Yoon, Suhn Young Im, Young Ho Kim |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Time Factors Lipopolysaccharide Biophysics Anti-Inflammatory Agents Oligosaccharides Inflammation Biology Nitric Oxide Biochemistry Microbiology Proinflammatory cytokine Nitric oxide Cell Line chemistry.chemical_compound Mice medicine Animals Secretion Molecular Biology Incubation Nitrites Chitosan Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Temperature Cell Biology chemistry Cell culture lipids (amino acids peptides and proteins) Tumor necrosis factor alpha medicine.symptom |
| Zdroj: | Biochemical and biophysical research communications. 358(3) |
| ISSN: | 0006-291X |
| Popis: | The focus of this study was to clarify the relation between the nitric oxide (NO) production and cytokine expression including tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), and also investigated the effect of COS on LPS stimuli from RAW 264.7 cell. The lipopolysaccharide (LPS) of Gram-negative bacteria induces the expression of cytokines and potent inducers of inflammatory cytokines such as TNF-alpha and IL-6. In this experiment, upon stimulation with increasing concentrations of chitosan, the LPS-stimulated TNF-alpha and IL-6 secretion was significantly recovered within the incubation media of RAW 264.7 cells. Consistently, RT-PCR with mRNA and Western blot with anti-cytokine antiserum including TNF-alpha and IL-6 showed that the amount of TNF-alpha and IL-6 secretion in the incubation media recovered with the concentration of chitosan. The LPS-stimulated NO secretion was significantly recovered within the 6h and 12h incubation media of RAW 264.7 cells, too. The recovery effect of chitosan on IL-6 and NO secretion may be induced via the stimulus of TNF-alpha in RAW 264.7 cell. These results once again suggest that chitosan oligosaccharide may have the anti-inflammatory effect via the stimulus of TNF-alpha in the LPS-stimulated inflammation in RAW 264.7 cells. |
| Databáze: | OpenAIRE |
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