Development of a radiolabeled β-human chorionic gonadotropin
| Autor: | Javad Garousi, Hassan Yousefnia, Mohammad Amin Hosseini, Mohammad Reza Khoshdel, Amir Reza Jalilian, Saeed Rajabifar, Daryoush Sardari |
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| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system Biodistribution medicine.medical_specialty medicine.drug_class Pharmaceutical Science Gallium High-performance liquid chromatography Human chorionic gonadotropin In vivo Internal medicine medicine Animals Humans Chorionic Gonadotropin beta Subunit Human Tissue Distribution Receptor Pharmacology Chromatography Chemistry Biological Transport General Medicine Pentetic Acid Rats Endocrinology Isotope Labeling Positron-Emission Tomography Specific activity Radiopharmaceuticals Gonadotropin Hormone |
| Zdroj: | Acta Pharmaceutica. 59:421-429 |
| ISSN: | 1846-9558 1330-0075 |
| DOI: | 10.2478/v10007-009-0035-6 |
| Popis: | Development of a radiolabeled β-human chorionic gonadotropinβ-Human chorionic gonadotropin (β-hCG) was successively labeled with [67Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM-1, labeling efficiency 80 %). Preliminaryin vivostudies (ID g-1, %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported β-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively. |
| Databáze: | OpenAIRE |
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