Heat Stress Factors Expressed during Seed Maturation Differentially Regulate Seed Longevity and Seedling Greening
| Autor: | José Luis Ruiz, Concepción Almoguera, Raúl Carranco, Juan Jordano, Pilar Prieto-Dapena |
|---|---|
| Přispěvatelé: | European Commission, Ministerio de Economía, Industria y Competitividad (España), Almoguera, Concepción, Prieto-Dapena, Pilar, Carranco, Raúl, Ruiz, José Luis, Jordano, Juan, Almoguera, Concepción [0000-0002-7961-6507], Prieto-Dapena, Pilar [0000-0002-2826-8688], Carranco, Raúl [0000-0001-9359-9793], Ruiz, José Luis [0000-0003-1901-1888], Jordano, Juan [0000-0002-2583-4453] |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine media_common.quotation_subject Plant Science 01 natural sciences Article Seedling greening 03 medical and health sciences Greening Seed maturation Photomorpho genesis HSF differential regulation Transcription factor Ecology Evolution Behavior and Systematics media_common Ecology Phytochrome biology Seed longevity Longevity Botany Differential regulation Differentialregulation biology.organism_classification Heat stress Cell biology photomorphogenesis 030104 developmental biology Seedling QK1-989 Photomorphogenesis 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 3, p 335 (2020) Digital.CSIC. Repositorio Institucional del CSIC instname Plants Volume 9 Issue 3 |
| ISSN: | 2223-7747 |
| Popis: | 13 páginas.- 7 figuras.- 36 referencias.- Supplementary Materials: The following are available online at http://www.mdpi.com/2223-7747/9/3/335/s1.- This article belongs to the Special Issue Genetics of Seed Germination and Growth Heat Stress Factor A9 (A9), a seed-specific transcription factor contributing to seed longevity, also enhances phytochrome-dependent seedling greening. The RNA-seq analyses of imbibed-seed transcripts here reported indicated potential additional effects of A9 on cryptochrome-mediated blue-light responses. These analyses also suggested that in contrast to the A9 effects on longevity, which require coactivation by additional factors as A4a, A9 alone might suffice for the enhancement of photomorphogenesis at the seedling stage. We found that upon its seed-specific overexpression, A9 indeed enhanced the expected blue-light responses. Comparative loss-of-function analyses of longevity and greening, performed by similar expression of dominant-negative and inactive forms of A9, not only confirmed the additional greening effects of A9, but also were consistent with A9 not requiring A4a (or additional factors) for the greening effects. Our results strongly indicate that A9 would differentially regulate seed longevity and photomorphogenesis at the seedling stage, A9 alone sufficing for both the phytochrome- and cryptochrome-dependent greening enhancement effects. This research was funded by FEDER (European Regional Development Fund) and by “Secretaría de Estado de Investigación, Desarrollo e Innovación. Ministerio de Economía, Industria y Competitividad” (Projects BIO2014-52303-R and BIO2017-82172-R) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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