Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation
| Autor: | P, Portoles, J, Rojo, A, Golby, M, Bonneville, S, Gromkowski, L, Greenbaum, C A, Janeway, D B, Murphy, K, Bottomly |
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| Rok vydání: | 1989 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD3 Complex T-Lymphocytes Immunology Receptors Antigen T-Cell Antigen-Presenting Cells Lymphocyte Activation Binding Competitive Cell Line Epitopes Immunoglobulin Fab Fragments Mice Mice Inbred AKR Species Specificity Cricetinae Animals Immunology and Allergy Mice Inbred BALB C Mice Inbred C3H Antibodies Monoclonal Precipitin Tests Clone Cells Rats Mice Inbred C57BL Mice Inbred DBA Rats Inbred Lew Mice Inbred CBA Binding Sites Antibody |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.142.12.4169 |
| Popis: | The TCR is comprised of two variable chains that confer specificity, called alpha:beta or gamma:delta, physically associated with five different molecules that comprise the complex known as CD3. Antibodies to this complex are very useful, as they react with all T lymphocytes. A rat mAb to mouse CD3 has been prepared. It reacts with 100% of T cells in all mouse strains tested but with no other cell type. It binds to the CD3 epsilon chain. This antibody activates cloned T cell lines and normal T cells, provided suitable accessory cells and signals are present. This antibody detects a determinant similar to but not identical with those detected by two previously reported hamster anti-CD3 epsilon antibodies. This antibody fixes C efficiently, and it is thus useful for depletion of T cells from bulk populations. Activation of T cells by one of the three different anti-CD3 epsilon antibodies was inhibited by the Fab fragment of anti-CD4, similar to the effects of anti-CD4 Fab on two previously reported anti-TCR V region antibodies that bind a CD3 epsilon-associated epitope. This further defines a site involving TCR V regions and CD3 epsilon with which CD4 appears to associate during T cell activation. |
| Databáze: | OpenAIRE |
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