Genetic diversity analyses of Lasiodiplodia theobromae on Morus alba and Agave sisalana based on RAPD and ISSR molecular markers
Autor: | Ji-guang Wei, X.B. Yang, Rong-shao Huang, Hong-hui Xie |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Population lcsh:QR1-502 Biology 01 natural sciences Microbiology lcsh:Microbiology 03 medical and health sciences RAPD Genetic variation Botany Lasiodiplodia theobromae education lcsh:QH301-705.5 education.field_of_study Genetic diversity Dendrogram ISSR genetic diversity Agave biology.organism_classification 030104 developmental biology Infectious Diseases Genetic distance lcsh:Biology (General) genetic variation 010606 plant biology & botany |
Zdroj: | Mycology, Vol 7, Iss 4, Pp 155-164 (2016) |
ISSN: | 2150-1211 2150-1203 |
Popis: | Genetic diversity of 23 Lasiodiplodia theobromae isolates on Morus alba and 6 isolates on Agave sisalana in Guangxi province, China, was studied by using random amplified polymorphic DNA and inter-simple sequence repeat molecular markers. Results of two molecular markers showed that the average percentage of polymorphic loci of all isolates was more than 93%. Both dendrograms of two molecular markers showed obvious relationship between groups and the geographical locations where those strains were collected, among which, the 23 isolates on M. alba were divided into 4 populations and the 6 isolates on A. sisalana were separated as a independent population. The average genetic identity and genetic distance of 5 populations were 0.7215, 0.3284 and 0.7915, 0.2347, respectively, which indicated that the genetic identity was high and the genetic distance was short in the 5 populations. Average value of the gene diversity index (H) and the Shannon’s information index (I) of 29 isolates were significantly higher than 5 populations which showed that genetic diversity of those isolates was richer than the populations and the degree of genetic differentiation of the isolates was higher. The Gst and Nm of 29 isolates were 0.4411, 0.6335 and 0.4756, 0.5513, respectively, which showed that the genetic diversity was rich in those isolates. |
Databáze: | OpenAIRE |
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