Kefir Peptides Exhibit Antidepressant-Like Activity in Mice Through the BDNF/TrkB Pathway
| Autor: | Hsiao-Ling Chen, Hsin-Shan Wu, Chih-Ching Yen, Ying-Wei Lan, Min-Yu Tu, Chuan-Mu Chen, Yu Tang Tung, Megan Ning-Yu Chan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Elevated plus maze
medicine.drug_class Tropomyosin receptor kinase B Pharmacology Anxiolytic Open field Rodent Diseases Mice 03 medical and health sciences Kefir Neurotrophic factors Genetics medicine Animals 030304 developmental biology 0303 health sciences Membrane Glycoproteins kefir peptides depressive-like behavior brain-derived neurotrophy factor tropomyosin receptor kinase B extracellular signal regulated kinase 1/2 Depression Chemistry Brain-Derived Neurotrophic Factor 0402 animal and dairy science 04 agricultural and veterinary sciences Protein-Tyrosine Kinases 040201 dairy & animal science Antidepressive Agents Tail suspension test Disease Models Animal Antidepressant Animal Science and Zoology Peptides Stress Psychological Food Science Behavioural despair test |
| DOI: | 10.5281/zenodo.4306994 |
| Popis: | Depression is a prevalent, stress-related mental disorder that can lead to serious psychiatric diseases with morbidity and high mortality. Although some functional fermented dairy drinks have promising anxiolytic and antidepressant effects, the mechanism is still not clear. To determine the antidepressant-like effect and the potential molecule mechanism of kefir peptides (KP), various behavioral tests, including the elevated plus maze test, open field test, forced swimming test, and tail suspension test, were used. Administration of 150 mg/kg KP in mice reduced the duration of immobility in the forced swimming test and tail suspension test, elevated the time spent in the open arm and center zone in the elevated plus maze test, and increased the total distance traveled, average speed, and time spent in the center zone in the open field test compared with the mock group. These results indicated that KP dramatically ameliorated the depression-like behaviors. Kefir peptides were further isolated and identified using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry, from which 3 peptides were identified and designated KFP-1, KFP-3, and KFP-5. Among these peptides, administration of KFP-3 (15 AA residues) remarkably decreased immobility time in the forced swimming test and increased mobility time in the tail suspension test. Therefore, KFP-3 may be the major active peptide with antidepressant activity in KP. Overexpression of brain-derived neurotrophic factor, phosphorylated tropomyosin receptor kinase B, and phosphorylated ERK1/2 protein levels could be detected in the hippocampus under KP administration. Therefore, we suggest that KP improves depressive-like behaviors by activating the brain-derived neurotrophic factor-phosphorylated tropomyosin receptor kinase B signaling pathway. Kefir peptides may serve as a new type of antidepressant dairy product and may provide potent antidepressant effects for clinical use. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|