Potential of Lactobacillus plantarum IBB3036 and Lactobacillus salivarius IBB3154 to persistence in chicken after in ovo delivery
| Autor: | Joanna Żylińska, Roman Krzysztof Górecki, Jarosław M. Cieśla, Krzysztof Gulewicz, Jacek Bardowski, Tamara Aleksandrzak-Piekarczyk, Weronika Puzia |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors Gastrointestinal Diseases medicine.medical_treatment In ovo Microbiology Polymerase Chain Reaction Bacterial Adhesion 03 medical and health sciences Feces Lactobacillus medicine Animals Food science Poultry Diseases Original Research 2. Zero hunger Gastrointestinal tract Microbial Viability 030102 biochemistry & molecular biology biology Prebiotic Lactobacillus salivarius Probiotics poultry food and beverages persistence biology.organism_classification Bacterial Load Gastrointestinal Tract 030104 developmental biology Osmolyte prebiotic Ligilactobacillus salivarius Chickens Lactobacillus plantarum |
| Zdroj: | MicrobiologyOpen |
| ISSN: | 2045-8827 |
| Popis: | The aim of this study was to characterize and compare selected Lactobacillus strains originating from different environments (cow milk and hen feces) with respect to their applicative potential to colonize gastrointestinal track of chickens before hatching from an egg. In vitro phenotypic characterization of lactobacilli strains included the investigation of the important prerequisites for persistence in gastrointestinal tract, such as a capability to survive in the presence of bile salts and at low pH, enzymatic and sugar metabolic profiles, adhesion abilities, and resistance to osmolytes, temperature, and antibiotics. Regarding the resistance of lactobacilli to most of the various stress factors tested, the milk isolate Lactobacillus plantarum IBB3036 showed better abilities than the chicken feces isolate Lactobacillus salivarius IBB3154. However, regarding the acidification tolerance and adherence ability, L. salivarius IBB3154 revealed better characteristics. Use of these two selected lactobacilli isolates together with proper prebiotics resulted in the preparation of two S1 and S2 bioformulations, which were injected in ovo into hen Cobb500 FF fertilized eggs. Furthermore, in vivo tests assessing the persistence of L. plantarum IBB3036 and L. salivarius IBB3154 in the chicken gastrointestinal tract was monitored by PCR‐based classical and quantitative techniques and revealed the presence of both strains in fecal samples collected 3 days after hatching. Subsequently, the number of L. salivarius IBB3154 increased significantly in the chicken intestine, whereas the presence of L. plantarum IBB3036 was gradually decreased. |
| Databáze: | OpenAIRE |
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