Increased T-bet to GATA-3 Ratio During Acute Allograft Rejection in the Rat Lung
| Autor: | Walter Weder, Martin Hersberger, L. Ji, Wolfgang Jungraithmayr, Lin Yang, Stephan Korom |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Graft Rejection
Male chemical and pharmacologic phenomena GATA3 Transcription Factor Andrology Th2 Cells Immune system Rats Inbred BN medicine Animals Humans Transplantation Homologous RNA Messenger Respiratory system Transcription factor DNA Primers Transplantation Lung Reverse Transcriptase Polymerase Chain Reaction business.industry Interleukin Th1 Cells Rats Transplantation Isogeneic medicine.anatomical_structure Rats Inbred Lew Allograft rejection Immunology Interleukin-2 Surgery Interleukin-4 T-Box Domain Proteins business Alloantigen recognition Lung Transplantation |
| Zdroj: | Transplantation Proceedings. 41:4316-4320 |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2009.08.057 |
| Popis: | Acute allograft rejection (AR) remains a major problem in solid organ transplantation. The pivotal mechanism hinges on alloantigen recognition by recipient T helper (T(h)) cells that differentiate into T(h)1 and T(h)2. This study investigated the association of mRNA levels of the transcription factors T-box expressed in T cells and GATA-binding protein 3 with the development of T(h)1/T(h)2-directed immune responses. We investigated the expression of T-bet and GATA-3 mRNA levels and the protein levels of their marker cytokines interleukin (IL)-2 and IL-4 in orthotopically transplanted rat lungs during AR. We observed a nonsignificant increase in T-bet expression following allografting at days 3 and 5 but there was a significant reduction in GATA-3 expression on day 5 compared with controls. The ratio of T-bet to GATA-3 expression showed a trend to increase at day 3 following allografting reaching significance at 5 days. These changes were associated with a significant increase in the expression of IL-2 over IL-4 on days 3 and 5. This study suggests that T(h)1 responses play a major role during AR in the rat lung, and that this differentiation can be monitored by measuring mRNA levels of T-bet and GATA-3. |
| Databáze: | OpenAIRE |
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