Tailored Hydrogels as Delivery Platforms for Conditioned Medium from Mesenchymal Stem Cells in a Model of Acute Colitis in Mice
| Autor: | Noemi Eiro, Leandro Avila, Roman Perez-Fernandez, Patricia Diaz-Rodriguez, Juan Sendon-Lago, Francisco J. Vizoso, Lorena Garcia-del Rio, Luis O. González, Mariana Landin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
human uterine cervical stem cells experimental colitis Pharmaceutical Science Inflammation Pharmacology Inflammatory bowel disease Article 03 medical and health sciences chemistry.chemical_compound Pharmacy and materia medica 0302 clinical medicine Mesalazine medicine conditioned medium delivery Colitis Acute colitis mucoadhesive and thermosensitive hydrogel business.industry Mesenchymal stem cell medicine.disease Ulcerative colitis digestive system diseases RS1-441 030104 developmental biology chemistry inflammation 030220 oncology & carcinogenesis medicine.symptom Stem cell business |
| Zdroj: | Pharmaceutics Volume 13 Issue 8 Pharmaceutics, Vol 13, Iss 1127, p 1127 (2021) |
| ISSN: | 1999-4923 |
| DOI: | 10.3390/pharmaceutics13081127 |
| Popis: | Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), is increasingly prevalent and current therapies are not completely effective. Mesenchymal stem cells are emerging as a promising therapeutic option. Here, the effect of local hydrogel application loaded with conditioned medium (CM) from human uterine cervical stem cells (hUCESC-CM) in an experimental acute colitis mice model has been evaluated. Colitis induction was carried out in C57BL/6 mice by dissolving dextran sulfate sodium (DSS) in drinking water for nine days. Ulcers were treated by rectal administration of either mesalazine (as positive control) or a mucoadhesive and thermosensitive hydrogel loaded with hUCESC-CM (H-hUCESC-CM). Body weight changes, colon length, and histopathological analysis were evaluated. In addition, pro-inflammatory TNF-α, IL-6, and IFN-γ mRNA levels were measured by qPCR. Treatment with H-hUCESC-CM inhibited body weight loss and colon shortening and induced a significant decrease in colon mucosa degeneration, as well as TNF-α, IFN-γ, and IL-6 mRNA levels. Results indicate that H-hUCESC-CM effectively alleviated DSS-induced colitis in mice, suggesting that H-hUCESC-CM may represent an attractive cell-free therapy for local treatment of IBD. |
| Databáze: | OpenAIRE |
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