tDCS Accelerates the Rehabilitation of MCAO-Induced Motor Function Deficits via Neurogenesis Modulated by the Notch1 Signaling Pathway
Autor: | Ling Guo, Lin Jiajin, Gui-Rong Ding, Zhang Keying, An Guangzhou, Zhao Tao, Jun-Ling Xing, Zhang Junping, Gang Rui, Zhou Yan |
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Rok vydání: | 2020 |
Předmět: |
Male
medicine.medical_treatment Neurogenesis Subventricular zone Striatum Motor Activity Transcranial Direct Current Stimulation Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Occlusion medicine Animals Notch1 signaling Receptor Notch1 030304 developmental biology Ischemic Stroke 0303 health sciences Rehabilitation Transcranial direct-current stimulation Behavior Animal business.industry Stroke Rehabilitation Infarction Middle Cerebral Artery General Medicine Recovery of Function Neural stem cell nervous system diseases Rats Disease Models Animal medicine.anatomical_structure nervous system business Neuroscience 030217 neurology & neurosurgery Signal Transduction |
Zdroj: | Neurorehabilitation and neural repair. 34(7) |
ISSN: | 1552-6844 |
Popis: | Background. Ischemic stroke carries a high mortality rate and is a leading cause of severe neurological disability. However, the efficacy of current therapeutic options remains limited. Objective. We aimed to investigate the treatment efficacy of transcranial direct current stimulation (tDCS) in motor function rehabilitation after ischemic stroke and explore the underlying mechanisms. Methods. Male Sprague-Dawley rats with epicranial electrodes were used to establish pathogenetic model through temporary right middle cerebral artery occlusion (MCAO). Subsequently, animals were randomly divided into 4 groups: MCAO + tDCS/sham tDCS, Control + tDCS/sham tDCS. Animals in the groups with tDCS underwent 10 days of cathodal tDCS totally (500 µA, 15 minutes, once a day). During and after tDCS treatment, the motor functions of the animals, ischemic damage area, proliferation and differentiation of neural stem cells (NSCs), and distribution, and protein expression of Notch1 signaling molecules were detected. Results. The rehabilitation of MCAO-induced motor function deficits was dramatically accelerated by tDCS treatment. NSC proliferation in the subventricular zone (SVZ) was significantly increased after MCAO surgery, and tDCS treatment promoted this process. Additionally, NSCs probably migrated from the SVZ to the ischemic striatum and then differentiated into neurons and oligodendrocytes after MCAO surgery, both of which processes were accelerated by tDCS treatment. Finally, tDCS treatment inhibited the activation of Notch1 signaling in NSCs in the ischemic striatum, which may be involved in NSC differentiation in the MCAO model. Conclusion. Our results suggest that tDCS may exert therapeutic efficacy after ischemic stroke in a regenerative medical perspective. |
Databáze: | OpenAIRE |
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