Involvement of Human Small Fragment Nuclease in the Resistance of Human Cells to UV-C-induced Cell Death¶
| Autor: | Chieko Wano, Nobuo Suzuki, Seiro Ito, Kazuko Kita, Ling Zhai, Akira Yamaura, Toshikazu Suzuki |
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| Rok vydání: | 2007 |
| Předmět: |
Exonucleases
Programmed cell death DNA repair Ultraviolet Rays Pyrimidine dimer Biology Biochemistry Cell Line chemistry.chemical_compound Gene expression Biomarkers Tumor Humans RNA Messenger Physical and Theoretical Chemistry Messenger RNA Cell Death Caspase 3 SMALL FRAGMENT NUCLEASE RNA General Medicine Molecular biology Neoplasm Proteins Kinetics chemistry 14-3-3 Proteins Pyrimidine Dimers Caspases Exoribonucleases RNA Interference DNA |
| Zdroj: | Photochemistry and Photobiology. 80:281-285 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/j.1751-1097.2004.tb00084.x |
| Popis: | Human small fragment nuclease (Sfn) is one of the cellular proteins that were reported to degrade small, single-stranded DNA and RNA. However, the biological role of Sfn in cellular response to various stressors such as UV-C (mainly 254 nm wavelength ultraviolet ray) remains unclear. We have examined whether modulation of human SFN gene expression affects cell survival capacity against UV-C-induced cell death, analyzing colony survival ability in UV-C-sensitive human RSa cells treated with short double-stranded RNA (siRNA) specific for SFN messenger RNA (mRNA). The expression levels of SFN mRNA in the siRNA-treated RSa cells decreased to about 15% compared with those in the control siRNA-treated cells. The siRNA-treated RSa cells showed lower colony survival and higher activity of caspase-3 after UV-C irradiation than the control siRNA-treated RSa cells. Furthermore, the removal capacity of cyclobutane pyrimidine dimers (CPD) in the siRNA-treated RSa cells decreased compared with the control siRNA-treated RSa cells. There was no difference in the colony survival and CPD removal capacity after UV-C irradiation between the control siRNA-treated RSa cells and mock-treated RSa cells. These results suggest that SFN expression is involved in resistance of RSa cells to UV-C-induced cell death through the roles it plays in the DNA repair process. |
| Databáze: | OpenAIRE |
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