Recombinant Baculovirus: A Flexible Drug Screening Platform for Chikungunya Virus

Autor: Chun-Chung Chen, Muhammed Muhsin Varikkodan, Tzong-Yuan Wu
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
viruses
membrane fusion
BacMam
medicine.disease_cause
Virus Replication
Cell Fusion
baculovirus
Viral Envelope Proteins
Sf9 Cells
Replicon
Chikungunya
Biology (General)
baicalin
Spectroscopy
Subgenomic mRNA
BacMam system
Cell fusion
virus diseases
General Medicine
Computer Science Applications
Chemistry
Baculoviridae
baicalein
QH301-705.5
030106 microbiology
Genetic Vectors
Green Fluorescent Proteins
Mosquito Vectors
Gene delivery
Biology
Antiviral Agents
Catalysis
Virus
Article
Inorganic Chemistry
03 medical and health sciences
antiviral drugs
medicine
Animals
drug screening
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
chikungunya virus
Organic Chemistry
fungi
Virology
High-Throughput Screening Assays
Internal ribosome entry site
030104 developmental biology
Chikungunya Fever
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 7891, p 7891 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 15
ISSN: 1661-6596
1422-0067
Popis: Chikungunya virus (CHIKV) is a mosquito-transmitted infectious agent that causes an endemic or epidemic outbreak(s) of Chikungunya fever that is reported in almost all countries. This virus is an intense global threat, due to its high rate of contagion and the lack of effective remedies. In this study, we developed two baculovirus expression vector system (BEVS)-based approaches for the screening of anti-CHIKV drugs in Spodoptera frugiperda insect (Sf21) cells and U-2OS cells. First, structural protein of CHIKV was co-expressed through BEVS and thereby induced cell fusion in Sf21 cells. We used an internal ribosome entry site (IRES) to co-express the green fluorescent protein (EGFP) for identifying these fusion events. The EGFP-positive Sf21 cells fused with each other and with uninfected cells to form syncytia. We identified that ursolic acid has potential anti-CHIKV activity in vitro, by using this approach. Second, BacMam virus-based gene delivery has been successfully applied for the transient expression of non-structural proteins with a subgenomic promoter-EGFP (SP-EGFP) cassette in U-2OS cells to act as an in vitro CHIKV replicon system. Our BacMam-based screening system has identified that the potential effects of baicalin and baicalein phytocompounds can inhibit the replicon activity of CHIKV in U-2OS cells. In conclusion, our results suggested that BEVS can be a potential tool for screening drugs against CHIKV.
Databáze: OpenAIRE
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