Reversed-phase liquid chromatographic method for the determination of 7-nitrobenz-2-oxa-1,3-diazol-4-yl-labelled lipid analogues
| Autor: | C. M. G. Frijters, Ronald P.J. Oude Elferink, Coosje J. Tuijn, Bart N. Zegers, Frans J. Hoek, Albert K. Groen |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Oxadiazoles
Ceramide Chromatography Chemistry Metabolite Extraction (chemistry) technology industry and agriculture General Chemistry Glucosylceramides High-performance liquid chromatography Fluorescence Sphingomyelins Serine Mice chemistry.chemical_compound Animals Bile Choline heterocyclic compounds lipids (amino acids peptides and proteins) Derivatization Chromatography Liquid Fluorescent Dyes |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications, 710(1-2), 9-16. Elsevier BV |
| ISSN: | 1387-2273 |
| DOI: | 10.1016/s0378-4347(98)00132-7 |
| Popis: | This paper reports the development of a dual column system for the simultaneous separation of fluorescent short-chain ceramide, 6-[(7-nitrobenz-2-oxa-1,3,-diazol-4-yl[NBD])amino]hexanoyl-sphingo sine and its metabolites, C6-NBD-sphingomyelin and C6-NBD-glucosylceramide, as well as the fluorescent derivatives of choline and serine phosphatides. The method enables the separation of these lipids in a single run on the basis of the polarity of their headgroups and hydrophobicity of their acyl backbone. The fluorescent properties of the NBD-label make it possible to quantitate small amounts of NBD-lipid analogues. The sensitivity of the presented method thus permits the use of small sample volumes and the determination of NBD-lipid analogues secreted into mouse bile directly, without prior extraction or concentration steps. |
| Databáze: | OpenAIRE |
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