Epicatechin Used in the Treatment of Intestinal Inflammatory Disease: An Analysis by Experimental Models

Autor: Paulo César de Paula Vasconcelos, Clélia Akiko Hiruma-Lima, Luiz Claudio Di Stasi, Cláudia Helena Pellizzon, Leonardo Noboru Seito
Přispěvatelé: Universidade Estadual Paulista (Unesp)
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Evidence-based Complementary and Alternative Medicine : eCAM
Evidence-Based Complementary and Alternative Medicine, Vol 2012 (2012)
Popis: Made available in DSpace on 2013-08-28T14:13:03Z (GMT). No. of bitstreams: 1 WOS000313444900001.pdf: 8337489 bytes, checksum: 61289a258f322f3d096913b8e27a030b (MD5) Made available in DSpace on 2013-09-30T18:36:57Z (GMT). No. of bitstreams: 2 WOS000313444900001.pdf: 8337489 bytes, checksum: 61289a258f322f3d096913b8e27a030b (MD5) WOS000313444900001.pdf.txt: 48969 bytes, checksum: c0fecdd931087aaedd7609b09b61dd40 (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:49:17Z No. of bitstreams: 2 WOS000313444900001.pdf: 8337489 bytes, checksum: 61289a258f322f3d096913b8e27a030b (MD5) WOS000313444900001.pdf.txt: 48969 bytes, checksum: c0fecdd931087aaedd7609b09b61dd40 (MD5) Made available in DSpace on 2014-05-20T13:49:17Z (GMT). No. of bitstreams: 2 WOS000313444900001.pdf: 8337489 bytes, checksum: 61289a258f322f3d096913b8e27a030b (MD5) WOS000313444900001.pdf.txt: 48969 bytes, checksum: c0fecdd931087aaedd7609b09b61dd40 (MD5) Previous issue date: 2012-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background. This study was pathway of (-)-epicatechin (EC) in the prevention and treatment of intestine inflammation in acute and chronic rat models. Methods. Intestine inflammation was induced in rats using TNBS. The morphological, inflammatory, immunohistochemical, and immunoblotting characteristics of colon samples were examined. The effects of EC were evaluated in an acute model at doses of 5, 10, 25, and 50 mg/kg by gavage for 5 days. The chronic colitis model was induced 1st day, and treated for 21 days. For the colitis relapse model, the induction was repeated on 14th. Results. EC10 and EC50 effectively reduced the lesion size, as assessed macroscopically; and confirmed by microscopy for EC10. The glutathione levels were higher in EC10 group but decreased COX-2 expression and increased cell proliferation (PC) were observed, indicating an anti-inflammatory activity and a proliferation-stimulating effect. In the chronic colitis model, EC10 showed lower macroscopic and microscopic lesion scores and increase in glutathione levels. As in the acute model, a decrease in COX-2 expression and an increase in PC in EC10, the chronic model this increase maybe by the pathway EGF expression. Conclusion. These results confirm the activity of EC as an antioxidant that reduces of the lesion and that has the potential to stimulate tissue healing, indicating useful for preventing and treating intestine inflammation. UNESP Univ Estadual Paulista, Biosci Inst, Morphol Dept, BR-18618970 Botucatu, SP, Brazil UNESP Univ Estadual Paulista, Biosci Inst, Pharmacol Dept, BR-18618970 Botucatu, SP, Brazil UNESP Univ Estadual Paulista, Biosci Inst, Physiol Dept, BR-18618970 Botucatu, SP, Brazil UNESP Univ Estadual Paulista, Biosci Inst, Morphol Dept, BR-18618970 Botucatu, SP, Brazil UNESP Univ Estadual Paulista, Biosci Inst, Pharmacol Dept, BR-18618970 Botucatu, SP, Brazil UNESP Univ Estadual Paulista, Biosci Inst, Physiol Dept, BR-18618970 Botucatu, SP, Brazil FAPESP: 02/05503-6 FAPESP: 06/55542-9 FAPESP: 07/53201-2 FAPESP: 09-52237-9 FAPESP: 10/08536-9
Databáze: OpenAIRE
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