GhHUB2, a ubiquitin ligase, is involved in cotton fiber development via the ubiquitin–26S proteasome pathway
| Autor: | Xin Li, Jiangli Dong, Ji Liu, Jie Deng, Chaojun Zhang, Hao Feng, Xueyan Zhang, Hong Chen, Tao Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteasome Endopeptidase Complex Physiology Ubiquitin-Protein Ligases fiber elongation Gossypium hirsutum Plant Science 01 natural sciences ubiquitin ligase 03 medical and health sciences Ubiquitin RNA interference Gene Expression Regulation Plant Monoubiquitination Fiber Transcription factor Plant Proteins Gossypium biology Chemistry Plants Genetically Modified Research Papers ubiquitin-26S proteasome pathway Cell biology Ubiquitin ligase 030104 developmental biology HUB2 Proteasome Crop Molecular Genetics secondary cell wall (SCW) biology.protein Secondary cell wall Cotton fiber 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | GhHUB2, a ubiquitin ligase, is involved in cotton fiber elongation and secondary cell wall deposition by degrading GhKNL1 via the ubiquitin–26S proteasome pathway, and thus demonstrates a novel function of HUB2 in plants. Cotton fibers, which are extremely elongated single cells of epidermal seed trichomes and have highly thickened cell walls, constitute the most important natural textile material worldwide. However, the regulation of fiber development is not well understood. Here, we report that GhHUB2, a functional homolog of AtHUB2, controls fiber elongation and secondary cell wall (SCW) deposition. GhHUB2 is ubiquitously expressed, including within fibers. Overexpression of GhHUB2 in cotton increased fiber length and SCW thickness, while RNAi knockdown of GhHUB2 resulted in shortened fibers and thinner cell walls. We found that GhHUB2 interacted with GhKNL1, a transcriptional repressor predominantly expressed in developing fibers, and that GhHUB2 ubiquitinated and degraded GhKNL1 via the ubiquitin-26S proteasome pathway. GhHUB2 negatively regulated GhKNL1 protein levels and lead to the disinhibition of genes such as GhXTH1, Gh1,3-β-G, GhCesA4, GhAGP4, GhCTL1, and GhCOBL4, thus promoting fiber elongation and enhancing SCW biosynthesis. We found that GhREV-08, a transcription factor that participates in SCW deposition and auxin signaling pathway, was a direct target of GhKNL1. In conclusion, our study uncovers a novel function of HUB2 in plants in addition to its monoubiquitination of H2B. Moreover, we provide evidence for control of the fiber development by the ubiquitin–26S proteasome pathway. |
| Databáze: | OpenAIRE |
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