The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins
| Autor: | Alberto Spanò, Daniela Bellarosa, Alessia Alberelli, Guido Antonelli, Carolina Scagnolari, Elisabetta Riva, R. Longo, Massimo Clementi, Simona Cicetti, Simona Trombetti |
|---|---|
| Přispěvatelé: | Scagnolari, C, Trombetti, S, Alberelli, A, Cicetti, S, Bellarosa, D, Longo, R, Spano, A, Riva, E, Clementi, Massimo, Antonelli, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
viruses
Short Communication 2 '-5 '-oligoadenylate synthetase 2′-5′-oligoadenylate synthetase interferon p56 severe acute respiratory syndrome coronavirus Gene Expression Stimulation Biology medicine.disease_cause Virus Replication Antiviral Agents Interferon-gamma Interferon Virology Chlorocebus aethiops medicine 2' 5'-Oligoadenylate Synthetase Animals RNA Messenger Respiratory system Beta (finance) Vero Cells Coronavirus RNA Proteins Drug Synergism Infectious Diseases Severe acute respiratory syndrome-related coronavirus Interferon Type I Vero cell Severe acute respiratory syndrome coronavirus medicine.drug |
| Zdroj: | Intervirology |
| Popis: | Interferon (IFN)-alpha, -beta and -gamma have been shown to be only marginally effective against severe acute respiratory syndrome coronavirus (SARS-CoV) replication in Vero cell lines. We investigated the combination of type I IFNs (IFN-alpha or -beta) and IFN-gamma for antiviral activity and found that such combinations synergistically inhibited SARS-CoV replication in Vero cells, using yield reduction assay and the isobologram and combination index methods of Chou and Talalay for evaluation. The highly synergistic anti-SARS-CoV action of type I IFNs and IFN-gamma parallels the marked increase in 2'-5'-oligoadenylate synthetase and p56 mRNAs following exposure in Vero cells to either IFN-alpha or -beta and IFN-gamma compared with the transcriptional levels obtained after stimulation with either IFN alone. These results demonstrate that SARS-CoV, although only moderately sensitive to the antiviral action of the individual types of IFN, is highly sensitive to a combination of type I and II IFNs, which suggests that such combinations may have potential in the treatment of SARS-CoV infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|