Boric acid exert anti-cancer effect in poorly differentiated hepatocellular carcinoma cells via inhibition of AKT signaling pathway
| Autor: | Erkan Kahraman, Erdem Göker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Carcinoma
Hepatocellular Hepatocellular carcinoma Apoptosis Growth Biochemistry Rodents Inorganic Chemistry Boric Acids Cell Line Tumor Humans Phosphorylation Migration Cell Proliferation Boron Cancer Liver Neoplasms Pi3k/Akt Boric acid Prognosis Dietary Boron Cancer drugs In-Vitro Human Prostate Molecular Medicine Proto-Oncogene Proteins c-akt Liver cancer Signal Transduction |
| Popis: | Background: The possible anti-cancer properties of boron, a trace element for humans, have been demonstrated in various experimental and epidemiological studies, although the effects of boron on liver cancer are unclear. In the present study we evaluate the effects of boric acid on the cell lines of hepatocellular carcinoma (HCC) of the liver, as the leading form of liver cancer, for which a poorly-differentiated HCC cell line (Mahlavu cell line) was used. Methods: The anti-cancer effect of boric acid was investigated with a cell viability assay, apoptosis analysis, cell migration analysis, cell morphology analysis, colony formation assay and 3D cell culture techniques. Also, the effect of boric acid on the AKT signaling pathway was determined through a western blot analysis. Results: Boric acid was found to reduce cell viability in a dose- and time-dependent manner, and decreased survival, colony formation ability, migration capability and HCC cell tumor spheroid growth in HCC cell lines, while also inducing apoptosis, autophagy and morphological alteration. Furthermore, boric acid inhibited AKT phosphorylation, and anticancer biological responses in HCC cells were observed only in cells in which AKT phosphorylation was suppressed by boric acid. Conclusion: Our results suggest that boric acid might be a promising therapeutic candidate in hepatocellular carcinoma via the inhibition of AKT signaling pathway. Academic Oncology Association from Turkey This study was funded by Academic Oncology Association from Turkey. |
| Databáze: | OpenAIRE |
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