Requirement for Phosphatidylinositol Transfer Protein in Epidermal Growth Factor Signaling

Autor: Shamshad Cockcroft, Simon Prosser, J. Justin Hsuan, Andrea Kauffmann-Zeh, Andrew Ball, Geraint M.H. Thomas, Emer Cunningham
Rok vydání: 1995
Předmět:
Zdroj: Scopus-Elsevier
ISSN: 1095-9203
0036-8075
DOI: 10.1126/science.7761838
Popis: Stimulation of phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis is a widespread mechanism for receptor-mediated signaling in eukaryotes. Cytosolic phosphatidylinositol transfer protein (PITP) is necessary for guanosine triphosphate (GTP)-dependent hydrolysis of PIP2 by phospholipase C-beta (PLC-beta), but the role of PITP is unclear. Stimulation of phospholipase C-gamma (PLC-gamma) in A431 human epidermoid carcinoma cells treated with epidermal growth factor (EGF) required PITP. Stimulation of PI-4 kinase in cells treated with EGF also required PITP. Coprecipitation studies revealed an EGF-dependent association of PITP with the EGF receptor, with PI-4 kinase, and with PLC-gamma.
Databáze: OpenAIRE