Requirement for Phosphatidylinositol Transfer Protein in Epidermal Growth Factor Signaling
Autor: | Shamshad Cockcroft, Simon Prosser, J. Justin Hsuan, Andrea Kauffmann-Zeh, Andrew Ball, Geraint M.H. Thomas, Emer Cunningham |
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Rok vydání: | 1995 |
Předmět: |
Biology
Phosphatidylinositols chemistry.chemical_compound Cytosol Epidermal growth factor Tumor Cells Cultured Animals Humans Phospholipid Transfer Proteins 1-Phosphatidylinositol 4-Kinase Phosphatidylinositol transfer protein Multidisciplinary Epidermal Growth Factor Phospholipase C Phospholipase C gamma Kinase Membrane Proteins Tyrosine phosphorylation Rats ErbB Receptors Isoenzymes Phosphotransferases (Alcohol Group Acceptor) Biochemistry Epidermoid carcinoma chemistry Type C Phospholipases Signal transduction Carrier Proteins A431 cells hormones hormone substitutes and hormone antagonists Protein Binding Signal Transduction |
Zdroj: | Scopus-Elsevier |
ISSN: | 1095-9203 0036-8075 |
DOI: | 10.1126/science.7761838 |
Popis: | Stimulation of phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis is a widespread mechanism for receptor-mediated signaling in eukaryotes. Cytosolic phosphatidylinositol transfer protein (PITP) is necessary for guanosine triphosphate (GTP)-dependent hydrolysis of PIP2 by phospholipase C-beta (PLC-beta), but the role of PITP is unclear. Stimulation of phospholipase C-gamma (PLC-gamma) in A431 human epidermoid carcinoma cells treated with epidermal growth factor (EGF) required PITP. Stimulation of PI-4 kinase in cells treated with EGF also required PITP. Coprecipitation studies revealed an EGF-dependent association of PITP with the EGF receptor, with PI-4 kinase, and with PLC-gamma. |
Databáze: | OpenAIRE |
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