Comparison of the Membrane Subproteomes during Growth of a New Pseudomonas Strain on Lysogeny Broth Medium, Glucose, and Phenol
| Autor: | Stavroula Markoutsa, Michael Karas, Anastasia Papadioti, Dimitrios G. Papasotiriou, Georgios Tsiotis, Bjoern Meyer |
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| Rok vydání: | 2008 |
| Předmět: |
Proteome
Molecular Sequence Data Cell Culture Techniques Biology Biochemistry chemistry.chemical_compound Bacterial Proteins Pseudomonas Lysogeny broth Inner membrane Electrophoresis Gel Two-Dimensional chemistry.chemical_classification Chromatography Phenol Cell Membrane Membrane Proteins Intracellular Membranes General Chemistry biology.organism_classification Culture Media Glucose Membrane chemistry Membrane protein Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Porin Bacterial outer membrane Aromatic hydrocarbon |
| Zdroj: | Journal of Proteome Research. 7:4278-4288 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr800192n |
| Popis: | Study of the bacterial membrane proteome is a field of growing interest in the research of nutrient transport and processing. Pseudomonas sp. strain phDV1, a Gram-negative bacterium selected for its ability to degrade aromatic compounds, was monitored under different growth substrate conditions, using lysogeny broth medium (LB), glucose, and phenol as sole carbon source. The aim of this study was to characterize the membrane subproteomes of the Pseudomonas strain by proteomic means to assess the protein composition of this subcellular compartments, which appears fundamental for the biodegradation of aromatic compounds. A total number of 129 different proteins have been identified by MALDI-TOF/TOF, 19 of which are membrane proteins that belong to the inner membrane and 10 that belong to the outer membrane. Two membrane proteins were only expressed in the presence of the aromatic substrate. We identified a membrane protein involved in aromatic hydrocarbon degradation as well as a probable porin which may, in fact, function as an aromatic compound-specific porin. Although the presence of different transporters have been reported for different aromatic compounds such as toluene and benzoic acid, to our knowledge, these are the first phenol-inducible membrane transporters identified. |
| Databáze: | OpenAIRE |
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