An Antigen Produced by Splicing of Noncontiguous Peptides in the Reverse Order
| Autor: | Suzanne M. Xuereb, Alexandre Dalet, Vincent Stroobant, Stanley R. Riddell, Marc A. Gavin, Edus H. Warren, Scott S. Tykodi, Nathalie Vigneron, Benoît Van den Eynde, Jeffrey K. Mito, Pierre Coulie |
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| Rok vydání: | 2006 |
| Předmět: |
Cytotoxicity
Immunologic Male Proteasome Endopeptidase Complex Amino Acid Motifs Molecular Sequence Data Antigen presentation Peptide Major histocompatibility complex Polymorphism Single Nucleotide Minor Histocompatibility Antigens Interferon-gamma Antigen Protein splicing Minor histocompatibility antigen Humans Protein Splicing Amino Acid Sequence Peptide sequence Alleles Cell Line Transformed chemistry.chemical_classification Antigen Presentation B-Lymphocytes Multidisciplinary HLA-A Antigens biology Nuclear Proteins Middle Aged Molecular biology Peptide Fragments Cell biology Electroporation Amino Acid Substitution chemistry RNA splicing biology.protein T-Lymphocytes Cytotoxic |
| Zdroj: | Science. 313:1444-1447 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | CD8-positive T lymphocytes recognize peptides that are usually derived from the degradation of cellular proteins and are presented by class I molecules of the major histocompatibility complex. Here we describe a human minor histocompatibility antigen created by a polymorphism in theSP110nuclear phosphoprotein gene. The antigenic peptide comprises two noncontiguous SP110 peptide segments spliced together in reverse order to that in which they occur in the predicted SP110 protein. The antigenic peptide could be produced in vitro by incubation of precursor peptides with highly purified 20Sproteasomes. Cutting and splicing probably occur within the proteasome by transpeptidation. |
| Databáze: | OpenAIRE |
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