Effect of Assembly Method on Nanoparticle Attachment Density, Growth Rate, and Motility of Nanoscale Bacteria Enabled Autonomous Drug Delivery System (NanoBEADS)
| Autor: | Ying Zhan, Austin Fergusson, Richey M. Davis, Lacey R. McNally, Bahareh Behkam |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology Chemistry Cancer therapy Motility Nanoparticle Nanotechnology 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification 03 medical and health sciences Drug delivery Nanomedicine 0210 nano-technology Nanoscopic scale Bacteria 030304 developmental biology |
| DOI: | 10.1101/867101 |
| Popis: | Microbial-mediated drug delivery systems have the potential to significantly enhance the efficacy of nanomedicine for cancer therapy through improved specificity and interstitial transport. The Nanoscale Bacteria-Enabled Autonomous Drug Delivery System (NanoBEADS) is a bacteria-based bio-hybrid drug delivery system designed to carry nanotherapeutics cargo deep into poorly vascularized cancerous tissue. The effect of bacteria-nanoparticle conjugation method and NanoBEADS assembly parameters (i.e., mixing method, volume, and duration) was investigated to maximize particle attachment density. The nanoparticle attachment capacity, viability, growth rate and motility of the original NanoBEADS and an antibody-free variant NanoBEADS were characterized and compared. It is found that the assembly parameters affect the attachment outcome and the binding mechanism impacts the attachment number, the growth rate and motility of NanoBEADS. The NanoBEADS platform provides an opportunity to load nanoparticles with different materials and sizes for applications beyond cancer therapy, such as imaging agents for high-resolution medical imaging. |
| Databáze: | OpenAIRE |
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