Establishment of a Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Immunosuppressant Levels in the Peripheral Blood Mononuclear Cells of Chinese Renal Transplant Recipients
| Autor: | Bing Chen, Hao-Qiang Shi, Pei-Jun Zhou, Hui-Min An, Jia-Qian Lu, Xiao-Hui Zhai, Kun Shao |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Adolescent Pharmacology Kidney 030226 pharmacology & pharmacy Peripheral blood mononuclear cell Tacrolimus Mycophenolic acid 03 medical and health sciences 0302 clinical medicine Asian People Pharmacokinetics Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Cyclosporin a medicine Humans Protein precipitation Pharmacology (medical) Whole blood Chemistry Mycophenolic Acid Kidney Transplantation Transplant Recipients Cyclosporine Leukocytes Mononuclear Female Drug Monitoring Immunosuppressive Agents Chromatography Liquid medicine.drug |
| Zdroj: | Therapeutic Drug Monitoring. 42:686-694 |
| ISSN: | 0163-4356 |
| DOI: | 10.1097/ftd.0000000000000765 |
| Popis: | BACKGROUND Monitoring immunosuppressant levels, such as mycophenolic acid (MPA), cyclosporin A (CsA), and tacrolimus (TAC), in peripheral blood mononuclear cells (PBMCs) could be useful in organ transplant patients administered individualized therapy. The authors developed a liquid chromatography-tandem mass spectrometry assay technique to simultaneously determine immunosuppressant levels in PBMCs and assess their pharmacokinetics in Chinese renal allograft recipients. METHODS PBMCs were isolated from the whole blood of 27 Chinese renal transplant patients using Ficoll-Paque Plus solution, and cell number was determined; acetonitrile treatment for protein precipitation, and gradient elution was performed on an Agilent Eclipse XDB-C18 column (3.5 μm, 2.1 × 100 mm) with mobile phase: water and methanol (containing 2 mM ammonium formate); flow rate: 0.3 mL·min. RESULTS The calibration curves of MPA, CsA, and TAC had a linear range (ng·mL): 0.098-39.2 (r = 0.9987), 0.255-102 (r = 0.9969), and 0.028-11.2 (r = 0.9993), respectively. The extraction effects, matrix effects, and mean relative recovery of these immunosuppressants were 70.4%-93.2%, 72.7%-96.5%, and 90.1%-112.4%, respectively. The within-day and between-day coefficients of variation were |
| Databáze: | OpenAIRE |
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