The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method
| Autor: | Simon Stables, Sinead McCarthy, Benjamin Ondruschka, Cristian Palmiere, Lina Woydt, Jack Garland, Kilak Kesha, Winston Philcox, Yeri Ahn, U. Da Broi, Rexson Tse, S Hensby-Bennet, Leo Lam |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Tryptase 01 natural sciences Pathology and Forensic Medicine Internal examination Aspirate 03 medical and health sciences Young Adult 0302 clinical medicine Cutdown Medicine Humans 030216 legal & forensic medicine External iliac vein Prospective Studies Sampling Prospective cohort study Anaphylaxis Forensic Pathology Aged Aged 80 and over Blood Specimen Collection biology business.industry Post mortem 010401 analytical chemistry Arterial Venous 2734 Gold standard (test) Venous blood Femoral Vein Middle Aged medicine.disease Constriction 0104 chemical sciences Peripheral Femoral Artery Anesthesia biology.protein Female Tryptases Autopsy business |
| Zdroj: | International journal of legal medicine. 133(5) |
| ISSN: | 1437-1596 |
| Popis: | Different sampling techniques can impact on post mortem tryptase levels. A previous study demonstrated significantly lower femoral post mortem total tryptase levels in samples collected via transcutaneous aspiration compared with directly sampling during internal examination. However, an outlier with high tryptase level was noted in one transcutaneous aspiration sample. This 6-month prospective study compared total post mortem tryptase levels between 21 paired aspirated venous and arterial femoral blood samples, and 19 paired aspirated and cutdown femoral venous blood samples in non-anaphylactic deaths only. No statistical differences were demonstrated between the different sampling methods. However, four outlier cases with higher tryptase levels in aspirated arterial and femoral cutdown samples compared with aspirated venous femoral samples were noted. The reasons for the outliers may be due to the bloods collected from these two methods being contaminated by central arterial and venous blood with high tryptase levels respectively. None of the aspirated venous femoral post mortem tryptase levels were above recognized post mortem tryptase cutoff to diagnose anaphylaxis. This study recommends aspirating blood samples from a clamped femoral/external iliac vein for post mortem tryptase analysis should be defined as the gold standard. Further study using the recommended sampling method on post mortem tryptase levels in non-anaphylactic and anaphylactic cases is warranted. |
| Databáze: | OpenAIRE |
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