Cold-Induced Accumulation of ω-3 Polyunsaturated Fatty Acid in a Liverwort,Marchantia polymorphaL
| Autor: | Haruka Kida, Kanji Ohyama, Tatsuro Hamada, Miho Takemura |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Fatty Acid Desaturases
Molecular Sequence Data Gene Expression Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Marchantia polymorpha Fatty Acids Omega-6 Fatty Acids Omega-3 Gene expression Marchantia Amino Acid Sequence RNA Messenger Molecular Biology Gene Phylogeny chemistry.chemical_classification Messenger RNA biology Organic Chemistry Fatty acid General Medicine biology.organism_classification Eicosapentaenoic acid Cold Temperature Isoenzymes chemistry Arachidonic acid Sequence Alignment Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 76:785-790 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.110915 |
| Popis: | The liverwort Marchantia polymorpha L. synthesizes various long-chain polyunsaturated fatty acids including arachidonic acid and eicosapentaenoic acid, neither of which is produced by higher plants. Here we report the effects of temperature on long-chain polyunsaturated fatty acid accumulation in the liverwort. The accumulation of ω-3 polyunsaturated fatty acids increased significantly as the growth temperature decreased. Specifically, the relative content of eicosapentaenoic acid to total fatty acids at 5 °C was approximately 3-fold higher than at 25 °C. On the other hand, the accumulation of ω-6 polyunsaturated fatty acids decreased at low temperatures. An analysis of gene expression indicated that the mRNA of the MpFAD3 gene for ER ω-3 desaturase increased significantly at 5 °C. These results indicate that in the liverwort the n-3 pathway was enhanced at low temperature, mainly via expression of the cold-induced ω-3 desaturase gene, leading to increased accumulation of eicosapentaenoic acid. |
| Databáze: | OpenAIRE |
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