The C-3 Epimer of 25-Hydroxyvitamin D3Is Present in Adult Serum
| Autor: | Michael A. Poquette, Gary L. Lensmeyer, Neil Binkley, Donald A. Wiebe |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Adolescent Stereochemistry Endocrinology Diabetes and Metabolism Clinical Biochemistry Stereoisomerism Context (language use) Tandem mass spectrometry Biochemistry Cohort Studies Young Adult chemistry.chemical_compound Endocrinology Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Humans Child Chromatography High Pressure Liquid Aged Calcifediol Aged 80 and over Chromatography Chemistry Biochemistry (medical) Infant Newborn Outcome measures Infant Reproducibility of Results Middle Aged Highly sensitive Child Preschool Female Epimer Blood Chemical Analysis |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 97:163-168 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jc.2011-0584 |
| Popis: | Epimers have identical molecular structure but differ in stereochemical configuration. It is widely believed that the C-3 epimer of 25-hydroxyvitamin D(3) [3-epi-25(OH)D(3)] is found only in neonates. However, this epimer was recently detected in a limited number of adults. The physiological importance of 3-epi-25(OH)D(3) is uncertain but might affect 25-hydroxyvitamin D test results and thereby reliability of the 25-hydroxyvitamin D(3) [25(OH)D(3)] measurement.This project describes development of a highly sensitive method for 3-epi-25(OH)D(3) measurement and establishes the prevalence of this epimer in adult clinical serum specimens. DESIGN, SETTING, PARTICIPANTS, AND MAIN OUTCOME MEASURE: Serum 25(OH)D(3), 3-epi-25(OH)D(3), and 25(OH)D(2) concentrations were determined in a cohort of patients (n = 214; age neonate to 80+ yr). High-performance liquid chromatography with ultraviolet detection and high-performance liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization equipped with cyanopropyl analytical columns were used to baseline separate and quantitate 25(OH)D(3), 3 epi-25(OH)D(3), and 25(OH)D(2).The C-3 epimer was detected in 212 of 214 (99%) of samples. Concentrations ranged from 1 to 93 ng/ml for 25(OH)D(3) and 0.1 to 23.7 ng/ml for 3-epi-25(OH)D(3). The relative amounts of epimer to 25(OH)D(3) ranged from 0 to 25.5% (mean 4.75%). The epimer amount increased as 25(OH)D(3) increased in a nonlinear mode. In sera with approximately the same 25(OH)D(3) concentration, the ratio of epimer to 25(OH)D(3) varied, e.g. at 25(OH)D(3) values of 20-22 ng/ml, the ratio varied from 2-8.5%.3-Epi-25(OH)D(3) is present in the majority of human serum specimens. Although this concentration is generally low, further work must investigate the impact of 3-epi-25(OH)D(3) on the various 25-hydroxyvitamin D assays and ultimately what information, if any, C-3 epimer measurement can provide clinically. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|