The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate)
| Autor: | Ana Maria Carmona-Ribeiro, Letícia Dias de Melo Carrasco, Denise Freitas Siqueira Petri, Luccas Missfeldt Sanches |
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| Rok vydání: | 2015 |
| Předmět: |
Staphylococcus aureus
Biocompatible polymer Dispersity Biomedical Engineering Allyl compound Pharmaceutical Science Medicine (miscellaneous) Emulsion polymerization Nanoparticle Antimicrobial nanoparticles Bioengineering Microbial Sensitivity Tests Applied Microbiology and Biotechnology Anti-Infective Agents Antimicrobial polymer Polymer chemistry Candida albicans Escherichia coli Humans Polymethyl Methacrylate Escherichia coli Infections BIOMATERIAIS Drug Carriers Chemistry Research Cationic polymerization technology industry and agriculture Candidiasis Particle characterization by light scattering and scanning electron microscopy Staphylococcal Infections Antimicrobial Allyl Compounds Quaternary Ammonium Compounds Poly (methylmethacrylate) Molecular Medicine Nanoparticles Drug carrier |
| Zdroj: | Journal of Nanobiotechnology Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1477-3155 |
| Popis: | Background Several cationic polymers exhibit a useful antimicrobial property, however the structure–activity relationship still requires a more complete investigation. The main objective of this work is the comparison between the antimicrobial activity and toxicity of free and immobilized poly (diallyldimethylammonium) chloride (PDDA) in biocompatible poly (methylmethacrylate) (PMMA) nanoparticles (NPs). Results NPs synthesis by emulsion polymerization is performed over a range of [PDDA] at two methylmethacrylate (MMA) concentrations. The PMMA/PDDA dispersions are characterized by dynamic light-scattering for sizing, polydispersity and zeta-potential analysis, scanning electron microscopy (SEM), plating plus colony forming unities (CFU) counting for determination of the minimal microbicidal concentrations (MMC) against Escherichia coli, Staphylococcus aureus and Candida albicans and hemolysis evaluation against mammalian erythrocytes. There is a high colloidal stability for the cationic PMMA/PDDA NPs over a range of [PDDA]. NPs diverse antimicrobial activity against the microorganisms reduces cell viability by eight-logs (E. coli), seven-logs (S. aureus) or two-logs (C. albicans). The NPs completely kill E. coli over a range of [PDDA] that are innocuous to the erythrocytes. Free PDDA antimicrobial activity is higher than the one observed for PDDA in the NPs. There is no PDDA induced-hemolysis at the MMC in contrast to the hemolytic effect of immobilized PDDA in the NPs. Hemolysis is higher than 15 % for immobilized PDDA at the MMC for S. aureus and C. albicans. Conclusions The mobility of the cationic antimicrobial polymer PDDA determines its access to the inner layers of the cell wall and the cell membrane, the major sites of PDDA antimicrobial action. PDDA freedom does matter for determining the antimicrobial activity at low PDDA concentrations and absence of hemolysis. Electronic supplementary material The online version of this article (doi:10.1186/s12951-015-0123-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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