Functional Expression of Gloeobacter Rhodopsin in PSI-Less Synechocystis sp. PCC6803
| Autor: | Filipe Branco dos Santos, Jos C. Arents, Klaas J. Hellingwerf, Christiane Funk, J. Merijn Schuurmans, Willem J. de Grip, Que Chen, Otilia Cheregi, Srividya Ganapathy |
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| Přispěvatelé: | Bacterial Cell Biology & Physiology (SILS, FNWI), Faculty of Science |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gloeobacter Histology lcsh:Biotechnology Biomedical Engineering Bioengineering 02 engineering and technology Photosynthetic efficiency growth stimulation oxygen evolution 03 medical and health sciences Electron transfer PSI-deletion Synechocystis lcsh:TP248.13-248.65 Proteorhodopsin Strain (chemistry) biology Chemiosmosis Chemistry Synechocystis Biochemistry and Molecular Biology 021001 nanoscience & nanotechnology biology.organism_classification retinal-based proton pump 030104 developmental biology Rhodopsin Biophysics biology.protein carotenoid metabolism sense organs 0210 nano-technology Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19] Biokemi och molekylärbiologi Biotechnology |
| Zdroj: | Frontiers in Bioengineering and Biotechnology, Vol 7 (2019) Frontiers in Bioengineering and Biotechnology, 7, 67 Frontiers in Bioengineering and Biotechnology Frontiers in Bioengineering and Biotechnology, 7:67. Frontiers Research Foundation Frontiers in Bioengineering and Biotechnology, 7 |
| ISSN: | 2296-4185 |
| Popis: | Contains fulltext : 205161.pdf (Publisher’s version ) (Open Access) The approach of providing an oxygenic photosynthetic organism with a cyclic electron transfer system, i.e., a far-red light-driven proton pump, is widely proposed to maximize photosynthetic efficiency via expanding the absorption spectrum of photosynthetically active radiation. As a first step in this approach, Gloeobacter rhodopsin was expressed in a PSI-deletion strain of Synechocystis sp. PCC6803. Functional expression of Gloeobacter rhodopsin, in contrast to Proteorhodopsin, did not stimulate the rate of photoheterotrophic growth of this Synechocystis strain, analyzed with growth rate measurements and competition experiments. Nevertheless, analysis of oxygen uptake and-production rates of the Gloeobacter rhodopsin-expressing strains, relative to the DeltaPSI control strain, confirm that the proton-pumping Gloeobacter rhodopsin provides the cells with additional capacity to generate proton motive force. Significantly, expression of the Gloeobacter rhodopsin did modulate levels of pigment formation in the transgenic strain. |
| Databáze: | OpenAIRE |
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