Characterization ofMycobacterium capraeIsolates from Europe by Mycobacterial Interspersed Repetitive Unit Genotyping
| Autor: | Artem Skrypnyk, Zeljko Cvetnic, Matjaz Ocepek, W. Erler, Maria Lodovica Pacciarini, Irmgard Moser, Anita Brandstätter, Maria Laura Boschiroli, Ludmila Naumann, Stefan Niemann, Tanja Kubica, Alicia Aranaz, Wolfgang M. Prodinger, Ivo Pavlik, G. Nagy |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Genotype Oligonucleotides Cattle Diseases Animals Wild Minisatellite Repeats Mycobacterium Animals Humans Tuberculosis Typing Genotyping Genetics Goat Diseases Phylogenetic tree biology Goats Mycobacteriology and Aerobic Actinomycetes History 20th Century biology.organism_classification Mycobacterium caprae Bacterial Typing Techniques Europe Interspersed Repetitive Sequences Mycobacterium tuberculosis complex DNA Transposable Elements Cattle Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 43:4984-4992 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.43.10.4984-4992.2005 |
| Popis: | Mycobacterium caprae, a recently defined member of theMycobacterium tuberculosiscomplex, causes tuberculosis among animals and, to a limited extent, in humans in several European countries. To characterizeM. capraein comparison with otherMycobacterium tuberculosiscomplex members and to evaluate genotyping methods for this species, we analyzed 232M. capraeisolates by mycobacterial interspersed repetitive unit (MIRU) genotyping and by spoligotyping. The isolates originated from 128 distinct epidemiological settings in 10 countries, spanning a period of 25 years. We found 78 different MIRU patterns (53 unique types and 25 clusters with group sizes from 2 to 9) but only 17 spoligotypes, giving Hunter-Gaston discriminatory indices of 0.941 (MIRU typing) and 0.665 (spoligotyping). For a subset of 103M. capraeisolates derived from outbreaks or endemic foci, MIRU genotyping and IS6110restriction fragment length polymorphism were compared and shown to provide similar results. MIRU loci 4, 26, and 31 were most discriminant inM. caprae, followed by loci 10 and 16, a combination which is different than those reported to discriminateM. bovisbest.M. capraeMIRU patterns together with published data were used for phylogenetic inference analysis employing the neighbor-joining method.M. capraeisolates were grouped together, closely related to the branches of classicalM. bovis,M. pinnipedii,M. microti, and ancestralM. tuberculosis, but apart from modernM. tuberculosis.The analysis did not reflect geographic patterns indicative of origin or spread ofM. caprae.Altogether, our data confirmM. capraeas a distinct phylogenetic lineage within theMycobacterium tuberculosiscomplex. |
| Databáze: | OpenAIRE |
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