| Autor: |
Kieran, Nicholas W., Suresh, Rahul, Dorion, Marie-France, MacDonald, Adam, Blain, Manon, Wen, Dingke, Fuh, Shih-Chieh, Ryan, Fari, Diaz, Roberto J., Stratton, Jo Anne, Ludwin, Samuel K., Sonnen, Joshua A., Antel, Jack, Healy, Luke M. |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.17982584 |
| Popis: |
Additional file 4: Figure S3. microRNAs are differentially expressed in primary human astrocytes undergoing different stresses. (A) Primary human astrocytes, oligodendrocytes, and microglia were separately cultured and analyzed for expression of canonically expressed genes of glial fibrillary acidic protein (GFAP), myelin basic protein (MBP) and ionized calcium binding adaptor molecule 1 (IBA1) by RT-qPCR. n = 3���4. (B) Primary human fetal astrocytes were immunostained with anti-GFAP, anti-O4, and anti-PU.1 antibodies. A CX7 automated microscope was used to quantify the percent positive astrocytes for each marker, n = 4. (C) Primary human astrocytes were treated in inflammatory (���I���) metabolic (���M���) or hypoxic (���H���) stress conditions induced by IL1b, glucose-free media, or a 1% oxygen chamber for 24 h compared to untreated control. RT-qPCR assessment of CXCL10, HMOX1, and MCT4 expression was measured to confirm the astrocytic response to inflammatory, metabolic, and hypoxic stress conditions, respectively, n = 4���6. (D) HeLa cells were left in normoxic (���N���) conditions for 48 h or were treated in 1% O2 (hypoxia, H) for the noted time period. After 2���48 h, the cells were removed and were immediately lysed with RIPA buffer for subsequent Western blot. n = 3. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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