Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis

Autor: Caner Akıl, Linh T. Tran, Magali Orhant-Prioux, Yohendran Baskaran, Yosuke Senju, Shuichi Takeda, Phatcharin Chotchuang, Duangkamon Muengsaen, Albert Schulte, Edward Manser, Laurent Blanchoin, Robert C. Robinson
Přispěvatelé: Institute of Molecular and Cell Biology, A*STAR, National University of Singapore (NUS), Research Institute for Interdisciplinary Science (RIIS), Okayama University, Okayama 700-8530, Japan, CytoMorphoLab, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Institut Universitaire d'Hématologie (IUH), Université Paris Diderot - Paris 7 (UPD7), JST CREST, grant number JPMJCR19S5, Japan, Japan Society for the Promotion of Science (JSPS), grant number JP20H00476 and 20K06589, Moore-Simons Project on the Origin of the Eukaryotic Cell, grant number GBMF9743, A*STAR, Singapore National Medical Research Council (NMRC grant OFIRG/0067/2018), Vidyasirimedhi Institute of Science and Technology (VISTEC), Wesco Scientific Promotion Foundation
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Communications Biology
Communications Biology, 2022, 5 (1), pp.890. ⟨10.1038/s42003-022-03783-1⟩
ISSN: 2399-3642
DOI: 10.1038/s42003-022-03783-1⟩
Popis: Charting the emergence of eukaryotic traits is important for understanding the characteristics of organisms that contributed to eukaryogenesis. Asgard archaea and eukaryotes are the only organisms known to possess regulated actin cytoskeletons. Here, we determined that gelsolins (2DGels) from Lokiarchaeota (Loki) and Heimdallarchaeota (Heim) are capable of regulating eukaryotic actin dynamics in vitro and when expressed in eukaryotic cells. The actin filament severing and capping, and actin monomer sequestering, functionalities of 2DGels are strictly calcium controlled. We determined the X-ray structures of Heim and Loki 2DGels bound actin monomers. Each structure possesses common and distinct calcium-binding sites. Loki2DGel has an unusual WH2-like motif (LVDV) between its two gelsolin domains, in which the aspartic acid coordinates a calcium ion at the interface with actin. We conclude that the calcium-regulated actin cytoskeleton predates eukaryogenesis and emerged in the predecessors of the last common ancestor of Loki, Heim and Thorarchaeota. Calcium-regulated actin filament assembly predates eukaryogenesis and was present in the last common ancestor of Asgard archaea Loki, Heim, and Thorarchaeota.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje