Antioxidant and Anti-inflammatory Properties of Algerian Thymelaea microphylla Coss. and Dur. Extracts
| Autor: | Antonio Speciale, Antonina Saija, Francesco Cimino, Saliha Dahamna, Khadidja Dehimi, Mariateresa Cristani, Roberto Raciti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Antioxidant
Oxygen radical absorbance capacity medicine.drug_class DPPH medicine.medical_treatment Pharmaceutical Science Pharmacology 01 natural sciences Anti-inflammatory Tumor necrosis factor‑α chemistry.chemical_compound medicinal plant Drug Discovery medicine oxidative stress Cyclooxygenase-2 Cyclooxygenase‑2 Thymelaea microphylla ABTS radical scavenger activity 010405 organic chemistry fungi food and beverages Catechin Free radical scavenger 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Biochemistry inflammation Original Article tumor necrosis factor-α Quercetin Cyclooxygenase‑2 Inflammation Medicinal plant Oxidative stress Radical scavenger activity Thymelaea microphylla Tumor necrosis factor‑α |
| Zdroj: | Pharmacognosy Magazine |
| ISSN: | 0976-4062 0973-1296 |
| Popis: | Background: Thymelaea microphylla Coss. et Dur. (Thymelaeaceae) (TM) is a rare medicinal plant endemic to Algeria. Leaves decoction is used in folk medicine for anticancer, anti-inflammatory, and antidiabetic properties. Objective: Herein, the antioxidant and anti-inflammatory properties of different extracts from leaves and flowers of Algerian TM were evaluated. Materials and Methods: The study was carried out by in vitro cell-free assays (antioxidant/radical properties), ex vivo experiments (inhibition of prostaglandin E2 and thromboxane B2 release in human whole blood) and in vitro experiments on cell systems (cytotoxicity on peripheral blood mononuclear cells, and protective effects on human vein endothelial cells exposed to TNF-α). Results: The acetone TM extract showed significant antioxidant properties and excellent anti-inflammatory and cyclooxygenase-inhibitory activity, together with lack of toxicity on normal human blood cells; furthermore, it was able to protect endothelial cells against dysfunction induced by TNF-α, as shown by decrease in cell death, e-selectin expression and leukocyte adhesion. Conclusion: On these bases, TM leaves and flowers appear to be a good source of bioactive compounds with significant antioxidant and antiinflammatory capability, and potentially effective in prevention and treatment of pathological conditions related to oxidative stress and inflammation, such as endothelial dysfunction. SUMMARY Thymelaea microphylla leaves and flowers are a good source of bioactive compounds with significant antioxidant/free radical scavenger and anti-inflammatory activity. The acetone extract from leaves and flowers of Algerian Thymelaea microphylla possesses excellent cyclooxygenase-inhibitory activity. This extract is able to protect against endothelial dysfunction, an early event in development of atherosclerosis and vascular diseases. Abbreviations used: TM: Thymelaea microphylla; BCB: Beta-carotene bleaching; AcE: Acetone extract; PGE2: Prostaglandin E2; TxB2: Thromboxane B2; FL: Fluorescein; Cat: Catechin; DPPH: 2,2-diphenyl-1-picrylhydrazyl; ABTS: 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid)+; Que: Quercetin; ORAC: Oxygen radical absorbance capacity; AAPH: 2,2'-azobis (2-methylpropionamidine)dihydrochloride; PMS/NADH: Phenazine methosulfate/nicotinamide adenine dinucleotide; HUVECs: Human umbilical vein endothelial cells. Antonio Speciale |
| Databáze: | OpenAIRE |
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