Resistance Mechanism of Chloramphenicol inStreptococcus haemolyticus, Streptococcus pneumoniaeandStreptococcus faecalis
| Autor: | Yoji Nakagawa, Sadao Miyamura, Yoshiyuki Nitahara, Hiroshi Ochiai, Michinori Terao |
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| Rok vydání: | 1977 |
| Předmět: |
Hot Temperature
Streptococcus pyogenes Immunology medicine.disease_cause Microbiology Chloramphenicol acetyltransferase Chloramphenicol Resistance Species Specificity Acetyltransferases Virology Streptococcus pneumoniae Enterococcus faecalis medicine Ammonium sulfate precipitation biology Streptococcus Chloramphenicol Drug Resistance Microbial Hydrogen-Ion Concentration biology.organism_classification Sephadex Enzyme Induction Bacteria medicine.drug |
| Zdroj: | Microbiology and Immunology. 21:69-76 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1977.tb02809.x |
| Popis: | The chloramphenicol resistance of Streptococcus haemolyticus, Streptococcus pneumoniae and Streptococcus faecalis isolated from clinical materials was proved to be due to an inactivating enzyme produced by these bacteria. The inactivated products of chloramphenicol were identified as 1-acetoxy, 3-acetoxy and 1,3-diacetoxy derivatives by thin-layer chromatography and infrared spectroscopy. The responsible enzyme was thus confirmed to be chloramphenicol acetyltransferase. The enzyme was inducible. It was partially purified by ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration on Sephadex G-150. The enzymes obtained from S. haemolyticus, S. pneumoniae and S. faecalis have been compared with the conclusion that they are identical with respect to molecular weight (approximately 75,000-80,000), optimum pH and heat stability. |
| Databáze: | OpenAIRE |
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