Deproteinization of Cortical Bone: Effects of Different Treatments
Autor: | Joanna McKittrick, Ekaterina Novitskaya, Peter Shyu, Yik Tung Tracy Ling, Sofia Lambert, Siyuan Pang, Kyungah Seo, Iwona Jasiuk, Kimberlin Zarate, Olivia A. Graeve, Frances Y. Su |
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Rok vydání: | 2018 |
Předmět: |
Thermogravimetric analysis
Swine Scanning electron microscope Endocrinology Diabetes and Metabolism 0206 medical engineering Composite number 02 engineering and technology Spectrum Analysis Raman Defatting symbols.namesake Crystallinity Endocrinology X-Ray Diffraction Phase (matter) Cortical Bone medicine Animals Orthopedics and Sports Medicine Minerals Histocytological Preparation Techniques Chromatography Chemistry 021001 nanoscience & nanotechnology 020601 biomedical engineering medicine.anatomical_structure Microscopy Electron Scanning symbols Cortical bone 0210 nano-technology Raman spectroscopy |
Zdroj: | Calcified Tissue International. 103:554-566 |
ISSN: | 1432-0827 0171-967X |
Popis: | Bone is a biological composite material having collagen and mineral as its main constituents. In order to better understand the arrangement of the mineral phase in bone, porcine cortical bone was deproteinized using different chemical treatments. This study aims to determine the best method to remove the protein constituent while preserving the mineral component. Chemicals used were H2O2, NaOCl, NaOH, and KOH, and the efficacy of deproteinization treatments was determined by thermogravimetric analysis and Raman spectroscopy. The structure of the residual mineral parts was examined using scanning electron microscopy. X-ray diffraction was used to confirm that the mineral component was not altered by the chemical treatments. NaOCl was found to be the most effective method for deproteinization and the mineral phase was self-standing, supporting the hypothesis that bone is an interpenetrating composite. Thermogravimetric analyses and Raman spectroscopy results showed the preservation of mineral crystallinity and presence of residual organic material after all chemical treatments. A defatting step, which has not previously been used in conjunction with deproteinization to isolate the mineral phase, was also used. Finally, Raman spectroscopy demonstrated that the inclusion of a defatting procedure resulted in the removal of some but not all residual protein in the bone. |
Databáze: | OpenAIRE |
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