UPTAKE OF HORSERADISH PEROXIDASE BY THE TESTIS AND EPIDIDYMIS OF MICE
| Autor: | Roberto E. Mancini, J. A. Aragon, Livia Lustig |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
Embryology medicine.medical_treatment Hamster Horseradish peroxidase Permeability Mice Endocrinology Rete testis Testis Methods medicine Animals Saline Epididymis Frozen section procedure Staining and Labeling biology Chemistry Obstetrics and Gynecology Cell Biology Blood proteins Molecular biology medicine.anatomical_structure Peroxidases Reproductive Medicine Injections Intravenous biology.protein Peroxidase |
| Zdroj: | Reproduction. 28:299-302 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0280299 |
| Popis: | Mancini, Vilar, Alvarez & Seiguer (1965) have demonstrated the passage through the blood\p=m-\testis barrier of labelled serum proteins injected intravenously into rats. Horseradish peroxidase was earlier introduced as a tracer by Straus (1957a) and applied to the ultrastructural level by Karnovsky (1965) and Graham & Karnovsky (1966). Sedar (1966a, b) reported, in two abstracts, that horseradish peroxidase injected intravenously into the hamster became localized in the epididymis and ductuli efferentes, and in the rete testis. Reddy & Svoboda (1967) observed that horseradish peroxidase injected intravenously into the rat was taken up by the cells of the testis. Recently, Fawcett, Leak & Heidger (1970), studying the permeability of the blood\p=m-\testis barrier in guinea-pigs, reported the uptake of horseradish peroxidase injected interstitially in the testis. The different results obtained by Fawcett et al. (1970) and Reddy & Svoboda (1967) on the uptake of horseradish peroxidase by the testis in different species, induced us to study the permeability of the blood\p=m-\testisbarrier and the epididymal tubules in the mouse. In the present report, light microscopic observations on the absorption of intravenously injected peroxidase by testes and epididymides of normal mice are described. The ultrastructural aspect of this study is in progress. Horseradish peroxidase (Sigma, type II), in a dose of 8 mg/mouse dissolved in 0-3 ml saline solution, was injected intravenously into the tails of twenty-five male mice (Rockland) varying from 25 to 30 g in weight. Animals were killed at 1,15, 30, 60, 90 and 120 min, and 3, 6, 9, 12, 18 and 24 hr following injec¬ tion. The test for peroxidase activity was performed as described by Graham & Karnovsky (1966) on 6-μ frozen sections of testis and epididymis. Free base, 3-3'diaminobenzidine, was used as the substrate for the peroxidase with peroxide in concentrations of 0-01% and 0-001%, the latter according to the method of Nakane & Pierce (1967). The following controls were used: (1) animals injected with saline solution and tested for peroxidase activity, (2) uninjected animals to test for the pre¬ sence of endogenous peroxidase, (3) animals injected with peroxidase but not tested for peroxidase activity. The sections were observed unstained or counterstained with Light Green. |
| Databáze: | OpenAIRE |
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