Laparoscopy-Assisted Creation of a Liver Failure Model in Pigs
| Autor: | Noriaki Tanaka, Naoya Kobayashi, Shinichi Matsumoto, Jorge David Rivas-Carrillo, Alejandro Soto-Guiterrez, Tsuyoshi Yamamoto, Takeshi Yuasa, Hirofumi Noguchi, Yong Chen, Nalu Navarro-Alvarez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Swine Biomedical Engineering Portal vein lcsh:Medicine chemistry.chemical_compound Hepatic Artery Occlusion Animals Medicine Laparoscopy Carbon Tetrachloride Transplantation medicine.diagnostic_test Portal Vein business.industry General surgery lcsh:R Liver failure Pig model Cell Biology Liver Artificial Hepatic ischemia Surgery Disease Models Animal chemistry Liver biopsy Hepatocytes Carbon tetrachloride business Liver Failure |
| Zdroj: | Cell Transplantation, Vol 17 (2008) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | We created a hepatic failure pig model that was suitable for the assessment of cell therapies, such as hepatocyte transplantation and bioartificial livers, using a laparoscopic surgical technique. In our model, all of three hepatic arteries were resected, 5, 7.5, or 10 ml of carbon tetrachloride (CCL4) was injected into the liver through the portal vein, and subsequently the portal vein was mechanically occluded for 30 min. After the portal occlusion was released, a liver biopsy was performed, and then the surgery was completed. Blood samples were regularly taken during the surgery in order to perform biochemical examinations. All of five pigs in which 5 ml of CCL4was infused recovered spontaneously and survived; in contrast, all of five pigs that received 10 ml CCL4died within 1.5 h after surgery. The pigs in which 7.5 ml CCL4was administered developed liver failure and survived for 6.4 h on average (±1.4 SD). Induction of liver failure with the use of 7.5 ml CCL4and 30-min hepatic ischemia fulfilled five of the six criteria that were proposed by Terblanche and Hickman: reversibility, reproducibility, death from liver failure, a therapeutic window, and a large-animal model. We believe that our model is the first report on creation of a reliable model for liver failure in pigs to assess the efficacy of liver-targeted cell therapies. |
| Databáze: | OpenAIRE |
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