Electrokinetic injection of DNA from gel micropads: basis for coupling polony technology with CE separation
| Autor: | Nikolai Abanshin, Sergey Pan'kov, Olga Kosobokova, Vyacheslav Khozikov, Georgiy Gudkov, Andriy Tsupryk, Vera Gorfinkel, Olga Somova, Marina Tcherevishnik, Dmitri Gavrilov, Andrey Stepukhovich |
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| Rok vydání: | 2007 |
| Předmět: |
Materials science
Capillary action Clinical Biochemistry Biochemistry Polymerase Chain Reaction Sensitivity and Specificity Polony Analytical Chemistry Electrophoresis Microchip Electrokinetic phenomena chemistry.chemical_compound Experimental proof Nanotechnology Monolith geography geography.geographical_feature_category Chromatography Base Sequence Reproducibility of Results DNA Equipment Design Sequence Analysis DNA Coupling (electronics) Electrophoresis chemistry Models Chemical Indicators and Reagents Spectrophotometry Ultraviolet Gels |
| Zdroj: | Electrophoresis. 28(21) |
| ISSN: | 0173-0835 |
| Popis: | We propose a novel method for electrokinetic injection of DNA samples into capillaries from nanoliter gel micropads, deposited on glass slides, which are coated with electroconducting film. Theoretical and experimental proof is presented for the proposed method. The method allows efficient and highly precise injection without physical contact between the gel pad and the capillary. Read length of more than 700 bp at Q20 has been reproducibly demonstrated in fused-silica capillaries using the proposed injection technique. Based on the obtained results we discuss a novel DNA sequencing system which combines DNA amplification and cycle sequencing in arrays of subnanoliter gel micropads and high-throughput electrophoretic separation in monolith multicapillary arrays. |
| Databáze: | OpenAIRE |
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