5-Aminolevulinic acid-mediated sonosensitization of rat RG2 glioma cells in vitro
| Autor: | Wojciech Secomski, Tamara Kujawska, Krzysztof Bilmin, Andrzej Nowicki, Paweł Grieb |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Survival lcsh:Medicine Apoptosis Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Line Tumor Animals MTT assay Viability assay sonodynamic therapy cell viability Protoporphyrin IX rat RG2 glioma cells Sonodynamic therapy lcsh:R Aminolevulinic Acid Glioma Rats 030104 developmental biology chemistry Cell culture 5-aminolevulinic acid 030220 oncology & carcinogenesis Cancer cell Biophysics Neurology (clinical) |
| Zdroj: | Folia Neuropathologica, Vol 54, Iss 3, Pp 234-240 (2016) |
| ISSN: | 1641-4640 |
| Popis: | Sonodynamic therapy (SDT) is a promising technique based on the ability of certain substances, called sonosensitizers, to sensitize cancer cells to non-thermal effects of low-energy ultrasound waves, allowing their destruction. Sonosensitization is thought to induce cell death by direct physical effects such as cavitation and acoustical streaming as well as by complementary chemical reactions generating oxygen free radicals. One of the promising sonosensitizers is 5-aminolevulinic acid (ALA) which upon selective uptake by cancer cells is metabolized and accumulated as protoporphyrin IX. The objective of the study was to describe ALA-mediated sonodynamic effects in vitro on a rat RG2 glioma cell line. Glioma cells, seeded at the bottom of 96-well plates and incubated with ALA (10 µg/ml) for 6 h, were exposed to the sinusoidal US pulses with a resonance frequency of 1 MHz, 1000 µs duration, 0.4 duty-cycle, and average acoustic power varying from 2 W to 6 W. Ultrasound waves were generated by a flat circular piezoelectric transducer with a diameter of 25 mm. Cell viability was determined by MTT assay. Structural cellular changes were visualized with a fluorescence microscope. Signs of cytotoxicity such as a decrease in cell viability, chromatin condensation and apoptosis were found. ALA-mediated SDT evokes cytotoxic effects of low intensity US on rat RG2 glioma cells in vitro . This cell line is indicated for further preclinical assessment of SDT in in vivo conditions. |
| Databáze: | OpenAIRE |
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