The protective effect of rosmarinic acid on myotube formation during myoblast differentiation under heat stress
| Autor: | Hui-Xia Li, Kun-Lin Chen, Zhi-Ping Lin, Yue Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Myoblast proliferation Cell Survival Muscle Fibers Skeletal Apoptosis MyoD Protective Agents Depsides Cell Line Myoblasts 03 medical and health sciences Mice 0302 clinical medicine Myocyte Animals RNA Messenger Myogenin MyoD Protein Myogenesis Chemistry Cell Differentiation Cell Biology General Medicine musculoskeletal system Cell biology 030104 developmental biology Gene Expression Regulation Cinnamates 030220 oncology & carcinogenesis Myogenic regulatory factors tissues C2C12 Developmental biology Heat-Shock Response Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 56(8) |
| ISSN: | 1543-706X |
| Popis: | High ambient temperature is one of the most important environmental factors that caused the reduction of livestock productivity and the increase of mortality. It has been shown that heat stress could affect the meat quality characteristics by physiological and metabolic perturbations in live livestock. Rosmarinic acid (RA) is a natural polyphenolic phytochemical compound that has many important biological activities, such as antioxidant, antimutagenic, and antitumor. The purpose of this study was to investigate the possible function and mechanism of RA on myoblast proliferation and differentiation under heat stress condition. The results showed that heat stress reduced the viability of myoblast and increased the percentage of apoptotic cells, and it also disrupted myotube formation by altering the expression of myogenic regulatory factors MyoD, myogenin, and MyHC. However, pretreatment of RA can protect C2C12 cells from heat stress-induced apoptosis, and it also increased the expression level of MyoD, myogenin, and MyHC under heat stress, which indicated that RA have protective effect on heat stress-caused failure of myotube formation during myoblast differentiation. Above all, our finding demonstrated that RA can promote the differentiation of C2C12 myoblast and maintain the formation of myotubes even under heat stress condition. |
| Databáze: | OpenAIRE |
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