Genomic structure and characterization of growth hormone receptors from golden pompano Trachinotus ovatus and their expression regulation by feed types
Autor: | Shigui Jiang, Huayang Guo, Dianchang Zhang, Yin-Yin Liang, Ke-Cheng Zhu, Bo Liu |
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Rok vydání: | 2019 |
Předmět: |
Fish Proteins
Physiology Sequence analysis Growth hormone receptor Aquatic Science Biochemistry Protein Structure Secondary Animals Amino Acid Sequence Cloning Molecular Gene Trachinotus ovatus Unsaturated fatty acid chemistry.chemical_classification Base Sequence biology Muscles Fishes Computational Biology food and beverages Receptors Somatotropin General Medicine biology.organism_classification Animal Feed Molecular biology Amino acid Intestines Transmembrane domain Gene Expression Regulation Liver chemistry Pompano Sequence Alignment Microsatellite Repeats |
Zdroj: | Fish Physiology and Biochemistry. 45:1845-1865 |
ISSN: | 1573-5168 0920-1742 |
Popis: | In this study, sequence analysis showed that ToGHR1 and ToGHR2 encoded polypeptides of 577 and 588 amino acids, respectively. Bioinformatics analysis showed that both ToGHR1 and ToGHR2 contain FN3 domains and transmembrane domains, which have glycosylation and phosphorylation sites. The exons of ToGHR1 and exons 4–10 of ToGHR2 are homologous to exons 2 and 4–9 in Homo sapiens genes, respectively. Only 3 SSR sites in ToGHR1 have SSR polymorphisms, and ToGHR2 has no SSR polymorphisms. ToGHR1 and ToGHR2 have high homology with GHR1 and GHR2 of many fish by BLAST. qRT-PCR was used to examine the expression profile of ToGHR mRNA in 12 normal liver and intestine tissue samples from 3 feed-type groups. The results showed that ToGHR is expressed in all 12 tissues, especially liver and muscle tissues, which showed higher ToGHR expression than that in other tissues (p |
Databáze: | OpenAIRE |
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