Intracellular endosymbiotic bacteria of Camponotus species (carpenter ants): systematics, evolution and ultrastructural characterization
| Autor: | Georg Krohne, Heike Deppisch, Doris Schröder, Malu Obermayer, Roy Gross, Werner Goebel, Bert Hölldobler, Erko Stackebrandt |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial food.ingredient Blochmannia DNA Ribosomal Polymerase Chain Reaction Microbiology food Phylogenetics RNA Ribosomal 16S Gram-Negative Bacteria Botany Animals Symbiosis Molecular Biology In Situ Hybridization Phylogeny Genetics Phylogenetic tree biology Ants Host (biology) Ovary fungi Bacteria Present biochemical phenomena metabolism and nutrition biology.organism_classification Biological Evolution Enterobacteriaceae Female Proteobacteria Bacteria |
| Zdroj: | Molecular Microbiology. 21:479-489 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1996.tb02557.x |
| Popis: | Intracellular endosymbiotic bacteria inherent to ants of the genus Camponotus were characterized. The bacteria were localized in bacteriocytes, which are specialized cells of both workers and queen ants; these cells are intercalated between epithelial cells of the midgut. The bacteriocytes show a different morphology from the normal epithelial cells and carry a large number of the rod-shaped Gram-negative bacteria free in the cytoplasm. The bacteria were never observed in the neighbouring epithelial cells, but they were found intracellularly in oocytes, strongly indicating a maternal transmission of the bacteria. The 16S DNA encoding rrs loci of the endosymbionts of four species of the genus Camponotus derived either from Germany (C. herculeanus and C. ligniperdus), North America (C. floridanus) or South America (C. rufipes) were cloned after polymerase chain reaction (PCR) amplification using oligonucleotides complementary to all so far known eubacterial rrs sequences. The DNA sequences of the rrs loci of the four endosymbionts were determined, and, using various genus- and species-specific oligonucleotides derived from variable regions in the rrs sequences, the identity of the bacteria present in the bacteriocytes and the ovarian cells was confirmed by PCR and in situ hybridization techniques. Comparison of the 16S DNA sequences with the available database showed the endosymbiotic bacteria to be members of the gamma-subclass of Proteobacteria. They formed a distinct taxonomic group, a sister taxon of the taxons defined by the tsetse fly and aphid endosymbionts. Within the gamma-subclass, the cluster of the ant, tsetse fly and aphid endosymbionts are placed adjacent to the family of Enterobacteriaceae. The evolutionary tree of the ant endosymbionts reflects the systematic classification and geographical distribution of their host insects, indicating an early co-evolution of the symbiotic partners and a vertical transmission of the bacteria. |
| Databáze: | OpenAIRE |
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