Comparative cytotoxic and genotoxic effects of permethrin and its nanometric form on human erythrocytes and lymphocytes in vitro
| Autor: | Amitava Mukherjee, Rajiv Sundaramoorthy, Yuvaraj Velusamy, A.P.B. Balaji, Natarajan Chandrasekaran |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Insecticides Erythrocytes Cell Survival Stereochemistry Lymphocyte Echinocyte 010501 environmental sciences Biology Toxicology medicine.disease_cause 01 natural sciences 03 medical and health sciences medicine Humans Lymphocytes Viability assay Cytotoxicity Cell Shape Cells Cultured Permethrin 0105 earth and related environmental sciences Dose-Response Relationship Drug General Medicine Molecular biology In vitro 030104 developmental biology medicine.anatomical_structure Micronucleus test Nanoparticles Micronucleus Genotoxicity |
| Zdroj: | Chemico-Biological Interactions. 257:119-124 |
| ISSN: | 0009-2797 |
| DOI: | 10.1016/j.cbi.2016.08.001 |
| Popis: | The research on the novel pesticides such as nanopesticides has become inevitable to control the mosquito population. Nanopermethrin (NP), one of such kind was formulated in pesticide loaded oil-in-water (o/w) microemulsion by rapid evaporation. Even though NP possess improved efficacy against the target pests, the toxicological investigation on the human or mammalian system remains unexplored. So, the present study focused on a comparative investigation of the cytotoxic and genotoxic effects of NP in vitro and its commercial parental bulk form of permethrin (BP) on human peripheral erythrocyte/lymphocyte by erythrocyte morphology analysis, cell viability assay, and cytokinesis-block micronucleus (CBMN) assay. The NP and BP concentrations (10, 25, 50 and 100 μg/ml) interacted with human blood cells, and the morphological changes were observed using a phase contrast microscope. The drastic increase of echinocyte was observed at 24, 48 and 72 h treatment as compared with the control. The cell viability studies have shown the significant decrease with increase in NP and BP concentration. CBMN study showed a series correlation in the number of micronuclei, bridge, bud, trinucleated and tetranucleated when interacted with different levels of NP and BP, as comparative to control *p < 0.05, **p < 0.001, ***p < 0.0001. |
| Databáze: | OpenAIRE |
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