Presence of collagen IV in the ciliary zonules of the human eye
| Autor: | Marja J. A. van Luyn, Leonoor I. Los, Roelofje J. van der Worp, Johanna M. M. Hooymans |
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| Přispěvatelé: | Restoring Organ Function by Means of Regenerative Medicine (REGENERATE) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Collagen Type IV 0301 basic medicine EXPRESSION Pathology medicine.medical_specialty Histology Adolescent EPITHELIUM Enzyme-Linked Immunosorbent Assay macromolecular substances engineering.material Eye Fibril Extracellular matrix 03 medical and health sciences Coating medicine collagen IV EXTRACELLULAR-MATRIX Humans BODY CHONDROITIN SULFATE PROTEOGLYCANS Child ciliary zonules Aged Microscopy 030102 biochemistry & molecular biology biology Chemistry MICROFIBRILS RAT EYE COMPONENTS Middle Aged Epithelium 030104 developmental biology medicine.anatomical_structure Polyclonal antibodies immunohistochemistry biology.protein engineering Biophysics Immunohistochemistry Human eye Anatomy Antibody |
| Zdroj: | Journal of Histochemistry & Cytochemistry, 52(6), 789-795 |
| ISSN: | 0022-1554 |
| DOI: | 10.1369/jhc.4A6267.2004 |
| Popis: | The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA. (J Histochem Cytochem 52:789–795, 2004) |
| Databáze: | OpenAIRE |
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