Immature Colon Carcinoma Transcript-1 (ICT1) Expression Correlates with Unfavorable Prognosis and Survival in Patients with Colorectal Cancer
| Autor: | Jianmin Xu, Meiling Ji, Xinyuan Lao, Wentao Tang, Xinyu Qin, Dexiang Zhu, Qingyang Feng, Guodong He, Pingping Xu |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Ribosomal Proteins 0301 basic medicine Oncology medicine.medical_specialty Cell Survival MAP Kinase Signaling System Colorectal cancer Apoptosis 03 medical and health sciences 0302 clinical medicine Cell Movement Surgical oncology Internal medicine medicine Humans Gene silencing Neoplasm Invasiveness In patient Gene Silencing Lung cancer Survival rate Aged Cell Proliferation Neoplasm Staging Cause of death business.industry Adenylate Kinase Carcinoma Proteins Cancer Middle Aged HCT116 Cells Prognosis medicine.disease digestive system diseases G2 Phase Cell Cycle Checkpoints Survival Rate 030104 developmental biology 030220 oncology & carcinogenesis Female Surgery Poly(ADP-ribose) Polymerases Colorectal Neoplasms business |
| Zdroj: | Annals of Surgical Oncology. 23:3924-3933 |
| ISSN: | 1534-4681 1068-9265 |
| DOI: | 10.1245/s10434-016-5305-1 |
| Popis: | Colorectal cancer (CRC) is the third leading cause of death from cancer worldwide. Immature colon carcinoma transcript-1 (ICT1) has been reported to be correlated with lung cancer; however, whether ICT1 is a functional gene in CRC, as well as the molecular mechanism underlying ICT1 mediation of colorectal-tumor formation, remains unknown.The expression of ICT1 was firstly determined by using immunohistochemistry in 861 CRC specimens. The correlation of ICT1 expression with clinicopathological parameters and the survival rate was analyzed. Furthermore, we investigated the effect of ICT1 silencing on CRC cell proliferation and migration by MTT, colony formation, flow cytometry, and transwell in vitro.ICT1 is highly expressed in a cohort of human CRCs, and that higher ICT1 expression may lead to reduced overall survival rate of CRC. Likewise, ICT1 silencing lowered the cell viability through cell-cycle arrest, inhibited cell migration, and induced apoptosis in CRC. We further revealed a novel mechanism in which ICT1 promoted CRC growth via the intracellular AMPK, SAPK/JNK, and PARP signaling pathways.Our data showed that ICT1 could be an important target for CRC diagnosis and treatment. |
| Databáze: | OpenAIRE |
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