Identification of target genes regulated by PAX3 and PAX3-FKHR in embryogenesis and alveolar rhabdomyosarcoma
| Autor: | Melisa C. Barber, Steve Ruben, Oana Tomescu, Thomas B. Friedman, Thomas D Barber, Frederic G. Barr |
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| Rok vydání: | 2002 |
| Předmět: |
Molecular Sequence Data
PAX3 Muscle Proteins Biology chemistry.chemical_compound Mice Genetics medicine Tumor Cells Cultured Animals Humans Paired Box Transcription Factors Binding site Gene Transcription factor PAX3 Transcription Factor Rhabdomyosarcoma Alveolar Gene Library Extracellular Matrix Proteins Nonmuscle Myosin Type IIB Vascular Endothelial Growth Factor Receptor-1 Myosin Heavy Chains Forkhead Box Protein O1 Gene Expression Regulation Developmental Membrane Proteins Forkhead Transcription Factors Transforming Growth Factor alpha musculoskeletal system medicine.disease Fusion protein DNA-Binding Proteins Gene Expression Regulation Neoplastic genomic DNA chemistry embryonic structures Gene Targeting Alveolar rhabdomyosarcoma Cell Adhesion Molecules DNA Transcription Factors |
| Zdroj: | Genomics. 79(3) |
| ISSN: | 0888-7543 |
| Popis: | PAX3 is a transcription factor important for neural, muscle, and facial development in vertebrates. To identify genes regulated by PAX3, we used a cyclic amplification and selection of targets (CASTing) strategy to isolate cis-regulatory elements bound by PAX3. CASTing libraries were constructed with mouse DNA fragments bound by mouse PAX3, and human genomic DNA fragments bound by human PAX3 and the fusion protein PAX3-FKHR. Approximately 1000 clones were sequenced from each of these three libraries. Numerous putative targets of PAX3 and PAX3-FKHR were identified and six genes, Itm2A, Fath, FLT1, TGFA, BVES, and EN2, were examined closely. The genomic DNA fragments near these genes contain PAX3 binding sites and confer PAX3-dependent regulation. The expression levels of these genes correlate with the PAX3 expression levels in mouse embryos or with PAX3-FKHR expression levels in rhabdomyosarcoma cell lines, and indicate they may be part of the PAX3 regulatory circuitry during embryogenesis and tumor formation. |
| Databáze: | OpenAIRE |
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