Effects of neuromedin B on steroidogenesis, cell proliferation and apoptosis in porcine Leydig cells
| Autor: | Jinlong Zhang, Zhiyu Ma, Wenna Qiao, Wenshao Wang, Sheng Yang, Juan Su, Na An, Ling Cheng, Ying Zhang, Xiang Li, Yanyan Feng, Zhihai Lei |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine endocrine system Neurokinin B Swine Apoptosis 03 medical and health sciences Endocrinology Proliferating Cell Nuclear Antigen medicine Animals Testosterone Secretion RNA Messenger Cyclin B1 Receptor Molecular Biology Cells Cultured Cell Proliferation bcl-2-Associated X Protein Leydig cell Caspase 3 urogenital system Cell growth Chemistry Cholesterol side-chain cleavage enzyme Leydig Cells Neuromedin B Cell biology Receptors Bombesin 030104 developmental biology medicine.anatomical_structure |
| Zdroj: | Journal of Molecular Endocrinology. 61:13-23 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1530/jme-17-0242 |
| Popis: | Neuromedin B (NMB), a mammalian bombesin-related peptide, has numerous physiological functions, including regulating hormone secretions, cell growth, and reproduction, by binding to its receptor (NMBR). In this study, we investigated the effects of NMB on testosterone secretion, steroidogenesis, cell proliferation, and apoptosis in cultured primary porcine Leydig cells. NMBR was mainly expressed in the Leydig cells of porcine testes, and a specific dose of NMB significantly promoted the secretion of testosterone in the primary Leydig cells; moreover, NMB increased the expression of mRNA and/or proteins of NMBR and steroidogenic mediators (steroidogenic acute regulatory (STAR), CYP11A1, and HSD3B1) in the Leydig cells. In addition, specific doses of NMB promoted the proliferation of Leydig cells and increased the expression of proliferating cell nuclear antigen and Cyclin B1 proteins, while suppressing Leydig cell apoptosis and decreasing BAX and Caspase-3 protein expression. These results suggest that the NMB/NMBR system might play an important role in regulating boar reproductive function by modulating steroidogenesis and/or cell growth in porcine Leydig cells. |
| Databáze: | OpenAIRE |
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