Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs

Autor: Andreas Oxenfarth, Mihajlo Novakovic, Robbin Schnieders, Eriks Kupče, Tali Scherf, Harald Schwalbe, J Tassilo Grün, Julia Wirmer-Bartoschek, Christian Richter, Lucio Frydman
Rok vydání: 2021
Předmět:
Zdroj: Angewandte Chemie International Edition
Angewandte Chemie (International Ed. in English)
Angewandte Chemie (Weinheim an Der Bergstrasse, Germany)
ISSN: 1433-7851
DOI: 10.1002/anie.202015948
Popis: 2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent‐driven exchanges to enhance NOE correlations between exchangeable and non‐exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross‐peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS‐CoV‐2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.
An approach to enhance the sensitivity of 2D NOESY correlations among labile protons is introduced and exemplified with experiments on RNA fragments derived from the SARS‐CoV‐2 genome and with other samples. The approach leads to experiments that are ca. 10‐fold faster and 3‐fold more sensitive than conventional counterparts. The mechanism of this sensitivity boost is explained, and its strengths and limitations are analyzed.
Databáze: OpenAIRE
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