Phagocytic Receptors Activate Syk and Src Signaling during Borrelia burgdorferi Phagocytosis
| Autor: | Alice Bedugnis, Tanja Petnicki-Ocwieja, Maria Ballesteros, Stephen C. Bunnell, Linden T. Hu, Tess L. Killpack, Lester Kobzik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Integrin beta Chains Phagocytosis Immunology Integrin Syk Biology Microbiology 03 medical and health sciences Mice Animals Syk Kinase Src family kinase Borrelia burgdorferi Receptors Immunologic Receptor Receptors Scavenger Lyme Disease Cellular Microbiology: Pathogen-Host Cell Molecular Interactions biology.organism_classification bacterial infections and mycoses Cell biology 030104 developmental biology Infectious Diseases src-Family Kinases biology.protein Parasitology Signal transduction Proto-oncogene tyrosine-protein kinase Src Signal Transduction |
| Popis: | Phagocytosis of the Lyme disease-causing pathogen Borrelia burgdorferi has been shown to be important for generating an inflammatory response to the pathogen. As a result, understanding the mechanisms of phagocytosis has been an area of great interest in the field of Lyme disease. Several cell surface receptors that participate in B. burgdorferi phagocytosis have been reported, including the scavenger receptor MARCO and integrin α3β1. We sought to define the mechanisms by which these receptors mediate phagocytosis and to identify signaling pathways activated downstream of these receptors upon contact with B. burgdorferi . We identified both Syk and Src signaling pathways as ones that participate in B. burgdorferi phagocytosis and the resulting cytokine activation. In our studies, we found that both MARCO and integrin β1 play a role in the activation of the Src kinase pathway. However, only integrin β1 participates in the activation of Syk. Interestingly, the integrin activates Syk without the help of the signaling adaptor Dap12 or FcRγ. Thus, we report that multiple pathways participate in B. burgdorferi internalization and that different cell surface receptors act simultaneously in cooperation and independently to mediate phagocytosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|