Vacuum Ultraviolet Laser Desorption/Ionization Mass Spectrometry Imaging of Single Cells with Submicron Craters
| Autor: | Zhaoying Wang, Feng Liu, Sichun Zhang, Xinrong Zhang, Hong-Yuan Chen, Jia Wang, Lesi Cai, Zhanping Li, Jian-Bin Pan, Yuxiang Mo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vacuum Ultraviolet Rays Mass spectrometry 01 natural sciences Fluence Mass spectrometry imaging Analytical Chemistry law.invention Ion 03 medical and health sciences Fragmentation (mass spectrometry) law Ionization Humans Chemistry business.industry 010401 analytical chemistry Epithelial Cells Laser 0104 chemical sciences 030104 developmental biology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mass spectrum Optoelectronics Single-Cell Analysis business HeLa Cells |
| Zdroj: | Analytical Chemistry. 90:10009-10015 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.8b02478 |
| Popis: | Mass spectrometry imaging (MSI) is a crucial label-free method to distinguish the localization patterns in single cells. MALDI-TOF MS and ToF-SIMS are now bearing the responsibility. However, MALDI-TOF MS is limited to micron spatial resolution and ToF-SIMS suffers from severe molecular fragmentation. Here, we proposed a new MSI methodology of vacuum ultraviolet laser desorption/ionization (VUVDI) with high spatial resolution, achieving higher ion yields and less fragmentation compared with ToF-SIMS at submicron level. The fluorescence image and mass spectrum of VUVDI were obtained simultaneously. In addition, the adjustable laser fluence acquired selective detection for different molecular and fragmental ions, thus realizing molecular identification. Furthermore, MSIs of single cells with submicron craters were presented. These results suggest VUVDI is a potential mass spectrometry method that provides a soft ionization source and submicron spatial resolution for molecular analysis in life science. |
| Databáze: | OpenAIRE |
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