Modulatory Effect of Trypanosoma cruzi Infective Stages in Different Dendritic Cell Populations in vitro
| Autor: | Marcel I. Ramirez, Estela Maria Lammel, Carolina Verónica Poncini, Brenda C. Gutierrez, Stella Maris González-Cappa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) T-Lymphocytes Trypanosoma cruzi 030106 microbiology Immunology T cells lcsh:QR1-502 Biology DENDRITIC CELLS Microbiology lcsh:Microbiology Cell Line purl.org/becyt/ford/1 [https] 03 medical and health sciences Cellular and Infection Microbiology Immune system Animals Parasite hosting CELL ACTIVATION dendritic cells purl.org/becyt/ford/1.6 [https] Cell Proliferation Antigen Presentation Mice Inbred C3H Tumor Necrosis Factor-alpha CYTOKINES TRYPANOSOMA CRUZI Dendritic cell Brief Research Report biology.organism_classification Acquired immune system cytokines In vitro Interleukin-10 cell activation Mice Inbred C57BL 030104 developmental biology Infectious Diseases Cell culture Langerhans Cells T CELLS Cell activation |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Cellular and Infection Microbiology, Vol 10 (2020) Frontiers in Cellular and Infection Microbiology |
| ISSN: | 2235-2988 |
| Popis: | Trypanosoma cruzi is a protozoan parasite that infects at least 7 million persons in the world (OMS, 2019). In endemic areas, infection normally occurs by vectorial transmission; however, outside, it normally happens by blood and includes congenital transmission. The persistence of T. cruzi during infection suggests the presence of immune evasion mechanisms and the modulation of the anti-parasite response to a profile incapable of eradicating the parasite. Dendritic cells (DCs) are a heterogeneous population of antigen-presenting cells (APCs) that patrol tissues with a key role in mediating the interface between the innate and adaptive immune response. Previous results from our lab and other groups have demonstrated that T. cruzi modulates the functional properties of DCs, in vitro and in vivo. During vectorial transmission, metacyclic (m) trypomastigotes (Tps) eliminated along with the insect feces reach the mucous membranes or injured skin. When transmission occurs by the hematic route, the parasite stage involved in the infection is the circulating or blood (b) Tp. Here, we studied in vitro the effect of both infective mTp and bTp in two different populations of DCs, bone marrow–derived DCs (BMDCs) and XS106, a cell line derived from epidermal DCs. Results demonstrated that the interaction of both Tps imparts a different effect in the functionality of these two populations of DCs, suggesting that the stage of T. cruzi and DC maturation status could define the immune response from the beginning of the ingress of the parasite, conditioning the course of the infection. Fil: Gutierrez, Brenda Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Lammel, Estela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Ramirez, Marcel Ivan. Fundación Oswaldo Cruz; Brasil. Universidade Federal do Paraná; Brasil Fil: González, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina Fil: Poncini, Carolina Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina |
| Databáze: | OpenAIRE |
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