Transcription of the caspase-14 gene in human epidermal keratinocytes requires AP-1 and NFκB
| Autor: | Jozef Ban, Susanne Karner, Erwin Tschachler, Maria Buchberger, Michael Mildner, Paul Mrass, Claudia Ballaun, Hanna Harant, Leopold Eckhart, Jürgen Bach |
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| Rok vydání: | 2008 |
| Předmět: |
Keratinocytes
Transcription Genetic JUNB Molecular Sequence Data Response element Biophysics Electrophoretic Mobility Shift Assay Biology Biochemistry Caspase 14 Humans Cloning Molecular Promoter Regions Genetic Molecular Biology Transcription factor Cells Cultured Reporter gene Binding Sites Base Sequence RELB NF-kappa B Promoter Cell Biology Molecular biology Transcription Factor AP-1 Epidermal Cells Gene Expression Regulation Chromosomal region Epidermis |
| Zdroj: | Biochemical and Biophysical Research Communications. 371:261-266 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.04.050 |
| Popis: | Caspase-14, a protease involved in skin barrier formation, is specifically expressed in epidermal keratinocytes (KCs). Here, we mapped three start sites of transcription of the human caspase-14 gene and analyzed the upstream chromosomal region for promoter activity. Reporter gene assays identified a core promoter region proximal to the first exon and a distal regulatory region which differentially suppressed promoter activity in KC and other cells. Sequence elements in the proximal promoter were bound by the transcription factors AP-1 (JunB, c-Jun, JunD, Fra-1 and Fra-2) and NFκB (p50 and RelB). Our data reveal the basic organization of the human caspase-14 promoter and suggest an important role of AP-1 and NFκB in the transcriptional control of caspase-14. |
| Databáze: | OpenAIRE |
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